Neurotrophin 3/TrkC-regulated proteins in the human medulloblastoma cell line DAOY

Mariella Gruber-Olipitz, Thomas Ströbel, Sung Ung Kang, Julius Paul Pradeep John, Michael A. Grotzer, Irene Slavc, Gert Lubec

Research output: Contribution to journalArticle

Abstract

Medulloblastoma (MB) is the most common malignant childhood brain tumor and high neurotrophin (NP) receptor TrkC mRNA expression was identified as a powerful independent predictor of favorable survival outcome. In order to determine downstream effector proteins of TrkC signaling, the MB cell line DAOY was stably transfected with a vector containing the full-length TrkC cDNA sequence or an empty vector control. A proteomic approach was used to search for expressional changes by two mass spectrometric methods and immunoblotting for validation of significant results. Multiple time points for up to 48 h following NP-3-induced TrkC receptor activation were chosen. Thirteen proteins from several pathways (nucleoside diphosphate kinase A, stathmin, valosin-containing protein, annexin A1, dihydropyrimidinase-related protein-3, DJ-1 protein, glutathione S-transferase P, lamin A/C, fascin, cofilin, vimentin, vinculin, and moesin) were differentially expressed and most have been shown to play a role in differentiation, migration, invasion, proliferation, apoptosis, drug resistance, or oncogenesis. Knowledge on effectors of TrkC signaling may represent a first useful step for the identification of marker candidates or reflecting probable pharmacological targets for specific treatment of MB.

Original languageEnglish (US)
Pages (from-to)540-549
Number of pages10
JournalELECTROPHORESIS
Volume30
Issue number3
DOIs
StatePublished - Apr 27 2009
Externally publishedYes

Keywords

  • DAOY
  • Medulloblastoma
  • Neurotrophin-3
  • Proteomics
  • TrkC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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  • Cite this

    Gruber-Olipitz, M., Ströbel, T., Kang, S. U., John, J. P. P., Grotzer, M. A., Slavc, I., & Lubec, G. (2009). Neurotrophin 3/TrkC-regulated proteins in the human medulloblastoma cell line DAOY. ELECTROPHORESIS, 30(3), 540-549. https://doi.org/10.1002/elps.200800325