Neurotrophic factors attenuate glutamate-induced accumulation of peroxides, elevation of intracellular Ca2+ concentration, and neurotoxicity and increase antioxidant enzyme activities in hippocampal neurons

Mark P. Mattson, Mark A. Lovell, Katsutoshi Furukawa, William R. Markesbery

Research output: Contribution to journalArticle

Abstract

Exposure of cultured rat hippocampal neurons to glutamate resulted in accumulation of cellular peroxides (measured using the dye 2,7-dichlorofluorescein). Peroxide accumulation was prevented by an N-methyl-D-aspartate (NMDA) receptor antagonist and by removal of extracellular Ca2+, indicating the involvement of NMDA receptor-induced Ca2+ influx in peroxide accumulation. Glutamate-induced reactive oxygen species contributed to loss Of Ca2+ homeostasis and excitotoxic injury because antioxidants (vitamin E, propyl gallate, and N-tertbutyl-a-phenylnitrone) suppressed glutamate-induced elevation of intracellular Ca2+ concentration ([Ca2+]i) and cell death. Basic fibroblast growth factor (bFGF), nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF), but not ciliary neurotrophic factor, each suppressed accumulation of peroxides induced by glutamate and protected neurons against excitotoxicity. bFGF, NGF, and BDNF each increased (to varying degrees) activity levels of Superoxide dismutases and glutathione reductase. NGF increased catalase activity, and BDNF increased glutathione peroxidase activity. The ability of the neurotrophic factors to suppress glutamate toxicity and glutamate-induced peroxide accumulation was attenuated by the tyrosine kinase inhibitor genistein, indicating the requirement for tyrosine phosphorylation in the neuroprotective signal transduction mechanism. The data suggest that glutamate toxicity involves peroxide production, which contributes to loss of Ca2+ homeostasis, and that induction of antioxidant defense systems is a mechanism underlying the [Ca2+]i-stabilizing and excitoprotective actions of neurotrophic factors.

Original languageEnglish (US)
Pages (from-to)1740-1751
Number of pages12
JournalJournal of Neurochemistry
Volume65
Issue number4
StatePublished - Oct 1995
Externally publishedYes

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Peroxides
Nerve Growth Factors
Enzyme activity
Neurons
Glutamic Acid
Antioxidants
Enzymes
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Fibroblast Growth Factor 2
N-Methyl-D-Aspartate Receptors
Toxicity
Homeostasis
Propyl Gallate
Ciliary Neurotrophic Factor
Signal transduction
Phosphorylation
Glutathione Reductase
Genistein
Cell death

Keywords

  • Brain-derived neurotrophic factor
  • Catalase
  • Dichlorofluorescein
  • Excitotoxicity
  • Fibroblast growth factor
  • Glutamate
  • Glutathione reductase
  • Peroxides
  • Receptor tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neurotrophic factors attenuate glutamate-induced accumulation of peroxides, elevation of intracellular Ca2+ concentration, and neurotoxicity and increase antioxidant enzyme activities in hippocampal neurons. / Mattson, Mark P.; Lovell, Mark A.; Furukawa, Katsutoshi; Markesbery, William R.

In: Journal of Neurochemistry, Vol. 65, No. 4, 10.1995, p. 1740-1751.

Research output: Contribution to journalArticle

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