TY - JOUR
T1 - Neurotransplantation in mice
T2 - The concorde-like position ensures minimal cell leakage and widespread distribution of cells transplanted into the cisterna magna
AU - Janowski, Miroslaw
AU - Kuzma-Kozakiewicz, Magdalena
AU - Binder, Donat
AU - Habisch, Hans Jörg
AU - Habich, Aleksandra
AU - Lukomska, Barbara
AU - Domanska-Janik, Krystyna
AU - Ludolph, Albert C.
AU - Storch, Alexander
N1 - Funding Information:
We appreciate the excellent technical assistance of Steffen Meier and Nancy Meyer. This study was supported by grants from the Ministry of Scientific Research and Information Technology of Poland grant no. K053/P05/2003 (MJ, MK, AH, BL), the Bundesministerium für Bildung und Forschung (Federal Ministry of Science and Technology), Polish-German Cooperation in Neuroscience Program (grant no. 01GZ0313; ACL, AS), and the Landesstiftung Baden-Württemberg (Förderprogramm ‘Adulte Stammzellen’ grant no. 37610; AS).
PY - 2008/1/10
Y1 - 2008/1/10
N2 - The access of transplanted cells to large areas of the CNS is of critical value for cell therapy of chronic diseases associated with widespread neurodegeneration. Intrathecal cell application can match this requirement. Here we describe an efficient method for cell injection into the cisterna magna and the assessment of the cell distribution within subarachnoidal space in mice. In order to maximize cell distribution we applied a "concord-like" position, where the cisterna magna is nearly the highest point of the animal's body. A drop of saline on the needle insertion site avoided the outflow of transplanted cells from subarachnoidal space with CSF during surgery. Twenty-four hours later the preparation of the CNS with an intact dura mater by a suitable dissection technique (described in detail) revealed approx. 80% of the injected cells (100,000 cells per animal) within the subarachnoidal space ranging from the skull base (olfactory nerve to premedullary cisterns) to the IV ventricle, and to both the ventral and dorsal surfaces of the spinal cord. Thus the "concorde-like" position proved to be very useful for intrathecal cell application leading to a widespread cell distribution within the subarachnoidal space.
AB - The access of transplanted cells to large areas of the CNS is of critical value for cell therapy of chronic diseases associated with widespread neurodegeneration. Intrathecal cell application can match this requirement. Here we describe an efficient method for cell injection into the cisterna magna and the assessment of the cell distribution within subarachnoidal space in mice. In order to maximize cell distribution we applied a "concord-like" position, where the cisterna magna is nearly the highest point of the animal's body. A drop of saline on the needle insertion site avoided the outflow of transplanted cells from subarachnoidal space with CSF during surgery. Twenty-four hours later the preparation of the CNS with an intact dura mater by a suitable dissection technique (described in detail) revealed approx. 80% of the injected cells (100,000 cells per animal) within the subarachnoidal space ranging from the skull base (olfactory nerve to premedullary cisterns) to the IV ventricle, and to both the ventral and dorsal surfaces of the spinal cord. Thus the "concorde-like" position proved to be very useful for intrathecal cell application leading to a widespread cell distribution within the subarachnoidal space.
KW - Cisterna magna
KW - Concorde-like position
KW - Dura preparation
KW - Neurotransplantation
KW - Stem cell therapy
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U2 - 10.1016/j.neulet.2007.10.050
DO - 10.1016/j.neulet.2007.10.050
M3 - Article
C2 - 18068302
AN - SCOPUS:37549070389
SN - 0304-3940
VL - 430
SP - 169
EP - 174
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -