Neurotransmitters selectively change the phosphorylation of H3 histone in identified neurons of the snail Helix lucorum

Alexey Shevelkin; V. P. Nikitin; V. V. Sherstnev

doi:10.1134/S1819712416030119

Neurotransmitters selectively change the phosphorylation of H3 histone in identified neurons of the snail Helix lucorum

Alexey Shevelkin, V. P. Nikitin, V. V. Sherstnev

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of serotonin and glutamate applications on H3 histone phosphorylation at serine 10 (Ser10, pHisH3) were immunohistochemically studied in the nuclei of L-RPa2-3 defensive behavior command neurons in the snail Helix lucorum. We found that simultaneous application of neurotransmitters induces biphasic changes in pHisH3-immunoreactivity in RPa2 neurons. The pHisH3 content increased at 30 min and decreased at 3 hours below the initial level, whereas in RPa3 neurons, a decrease in pHisH3 at 3 h occurred. Changes in pHisH3-immunoreactivity were not found in LPa2 and LPa3 neurons during the 7 hours of the experiment. Alterations of pHisH3-immunoreactivity in neural nuclei coincided with significant changes in colocalization with DNA. Our results demonstrate neuron-specific changes in genome activity in the neural nuclei of identified neurons in a cell model of a simple form of learning.

Original language	English (US)
Pages (from-to)	190-194
Number of pages	5
Journal	Neurochemical Journal
Volume	10
Issue number	3
DOIs	https://doi.org/10.1134/S1819712416030119
State	Published - Jul 1 2016
Externally published	Yes

Keywords

defensive behavior command neuron
glutamate
neuronal plasticity
phosphorylated histone H3 (Ser10)
serotonin
snail

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cellular and Molecular Neuroscience

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10.1134/S1819712416030119

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title = "Neurotransmitters selectively change the phosphorylation of H3 histone in identified neurons of the snail Helix lucorum",

abstract = "The effects of serotonin and glutamate applications on H3 histone phosphorylation at serine 10 (Ser10, pHisH3) were immunohistochemically studied in the nuclei of L-RPa2-3 defensive behavior command neurons in the snail Helix lucorum. We found that simultaneous application of neurotransmitters induces biphasic changes in pHisH3-immunoreactivity in RPa2 neurons. The pHisH3 content increased at 30 min and decreased at 3 hours below the initial level, whereas in RPa3 neurons, a decrease in pHisH3 at 3 h occurred. Changes in pHisH3-immunoreactivity were not found in LPa2 and LPa3 neurons during the 7 hours of the experiment. Alterations of pHisH3-immunoreactivity in neural nuclei coincided with significant changes in colocalization with DNA. Our results demonstrate neuron-specific changes in genome activity in the neural nuclei of identified neurons in a cell model of a simple form of learning.",

keywords = "defensive behavior command neuron, glutamate, neuronal plasticity, phosphorylated histone H3 (Ser10), serotonin, snail",

author = "Alexey Shevelkin and Nikitin, {V. P.} and Sherstnev, {V. V.}",

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AU - Shevelkin, Alexey

AU - Nikitin, V. P.

AU - Sherstnev, V. V.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - The effects of serotonin and glutamate applications on H3 histone phosphorylation at serine 10 (Ser10, pHisH3) were immunohistochemically studied in the nuclei of L-RPa2-3 defensive behavior command neurons in the snail Helix lucorum. We found that simultaneous application of neurotransmitters induces biphasic changes in pHisH3-immunoreactivity in RPa2 neurons. The pHisH3 content increased at 30 min and decreased at 3 hours below the initial level, whereas in RPa3 neurons, a decrease in pHisH3 at 3 h occurred. Changes in pHisH3-immunoreactivity were not found in LPa2 and LPa3 neurons during the 7 hours of the experiment. Alterations of pHisH3-immunoreactivity in neural nuclei coincided with significant changes in colocalization with DNA. Our results demonstrate neuron-specific changes in genome activity in the neural nuclei of identified neurons in a cell model of a simple form of learning.

AB - The effects of serotonin and glutamate applications on H3 histone phosphorylation at serine 10 (Ser10, pHisH3) were immunohistochemically studied in the nuclei of L-RPa2-3 defensive behavior command neurons in the snail Helix lucorum. We found that simultaneous application of neurotransmitters induces biphasic changes in pHisH3-immunoreactivity in RPa2 neurons. The pHisH3 content increased at 30 min and decreased at 3 hours below the initial level, whereas in RPa3 neurons, a decrease in pHisH3 at 3 h occurred. Changes in pHisH3-immunoreactivity were not found in LPa2 and LPa3 neurons during the 7 hours of the experiment. Alterations of pHisH3-immunoreactivity in neural nuclei coincided with significant changes in colocalization with DNA. Our results demonstrate neuron-specific changes in genome activity in the neural nuclei of identified neurons in a cell model of a simple form of learning.

KW - defensive behavior command neuron

KW - glutamate

KW - neuronal plasticity

KW - phosphorylated histone H3 (Ser10)

KW - serotonin

KW - snail

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JF - Neurochemical Journal

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Neurotransmitters selectively change the phosphorylation of H3 histone in identified neurons of the snail Helix lucorum

Abstract

Keywords

ASJC Scopus subject areas

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