Neurotransmitters selectively change the phosphorylation of H3 histone in identified neurons of the snail Helix lucorum

Alexey Shevelkin, V. P. Nikitin, V. V. Sherstnev

Research output: Contribution to journalArticle

Abstract

The effects of serotonin and glutamate applications on H3 histone phosphorylation at serine 10 (Ser10, pHisH3) were immunohistochemically studied in the nuclei of L-RPa2-3 defensive behavior command neurons in the snail Helix lucorum. We found that simultaneous application of neurotransmitters induces biphasic changes in pHisH3-immunoreactivity in RPa2 neurons. The pHisH3 content increased at 30 min and decreased at 3 hours below the initial level, whereas in RPa3 neurons, a decrease in pHisH3 at 3 h occurred. Changes in pHisH3-immunoreactivity were not found in LPa2 and LPa3 neurons during the 7 hours of the experiment. Alterations of pHisH3-immunoreactivity in neural nuclei coincided with significant changes in colocalization with DNA. Our results demonstrate neuron-specific changes in genome activity in the neural nuclei of identified neurons in a cell model of a simple form of learning.

Original languageEnglish (US)
Pages (from-to)190-194
Number of pages5
JournalNeurochemical Journal
Volume10
Issue number3
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Keywords

  • defensive behavior command neuron
  • glutamate
  • neuronal plasticity
  • phosphorylated histone H3 (Ser10)
  • serotonin
  • snail

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cellular and Molecular Neuroscience

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