Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells

Hiromichi Kumagai, Hisato Sakamoto, Sandra Guggino, Charles R. Filburn, Bertram Sacktor

Research output: Contribution to journalArticle


The nervous system may play a role in regulation of bone metabolism. The effects of norepinephrine(NE), vasoactive intestinal peptide(VIP), and ATP on cytosolic Ca2+ were assessed in a rat osteoblast-like osteosarcoma cell line (UMR-106) responsive to PTH. All three transmitters transiently increased Ca2+, with ATP≫PTH>NE=VIP, and then caused sustained increases in Ca2+. The ATP-induced transient resulted from mobilization of intracellular Ca2+ store, while NE and VIP-induced transients also involved influx of Ca2+. Later sustained increases by all agonists were dependent upon extracellular Ca2+. Release of intracellular Ca2+ by ATP was associated with a marked increase in IP3 but without a significant change in cAMP. NE, VIP, and ATP, through regulation of Ca2+ metabolism, may be involved in various osteoporotic conditions.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalCalcified Tissue International
Issue number4
StatePublished - Jul 1 1989


  • Bone resorption
  • cytosolic Ca
  • neurotransmitter
  • purinergic receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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    Kumagai, H., Sakamoto, H., Guggino, S., Filburn, C. R., & Sacktor, B. (1989). Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells. Calcified Tissue International, 45(4), 251-254.