Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells

Hiromichi Kumagai; Hisato Sakamoto; Sandra Guggino; Charles R. Filburn; Bertram Sacktor

doi:10.1007/BF02556045

Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells

Hiromichi Kumagai, Hisato Sakamoto, Sandra Guggino, Charles R. Filburn, Bertram Sacktor

School of Medicine

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The nervous system may play a role in regulation of bone metabolism. The effects of norepinephrine(NE), vasoactive intestinal peptide(VIP), and ATP on cytosolic Ca²⁺ were assessed in a rat osteoblast-like osteosarcoma cell line (UMR-106) responsive to PTH. All three transmitters transiently increased Ca²⁺, with ATP≫PTH>NE=VIP, and then caused sustained increases in Ca²⁺. The ATP-induced transient resulted from mobilization of intracellular Ca²⁺ store, while NE and VIP-induced transients also involved influx of Ca²⁺. Later sustained increases by all agonists were dependent upon extracellular Ca²⁺. Release of intracellular Ca²⁺ by ATP was associated with a marked increase in IP3 but without a significant change in cAMP. NE, VIP, and ATP, through regulation of Ca²⁺ metabolism, may be involved in various osteoporotic conditions.

Original language	English (US)
Pages (from-to)	251-254
Number of pages	4
Journal	Calcified Tissue International
Volume	45
Issue number	4
DOIs	https://doi.org/10.1007/BF02556045
State	Published - Jul 1 1989

Keywords

Bone resorption
cytosolic Ca
neurotransmitter
purinergic receptor

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Orthopedics and Sports Medicine
Endocrinology

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10.1007/BF02556045

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abstract = "The nervous system may play a role in regulation of bone metabolism. The effects of norepinephrine(NE), vasoactive intestinal peptide(VIP), and ATP on cytosolic Ca2+ were assessed in a rat osteoblast-like osteosarcoma cell line (UMR-106) responsive to PTH. All three transmitters transiently increased Ca2+, with ATP≫PTH>NE=VIP, and then caused sustained increases in Ca2+. The ATP-induced transient resulted from mobilization of intracellular Ca2+ store, while NE and VIP-induced transients also involved influx of Ca2+. Later sustained increases by all agonists were dependent upon extracellular Ca2+. Release of intracellular Ca2+ by ATP was associated with a marked increase in IP3 but without a significant change in cAMP. NE, VIP, and ATP, through regulation of Ca2+ metabolism, may be involved in various osteoporotic conditions.",

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AU - Kumagai, Hiromichi

AU - Sakamoto, Hisato

AU - Guggino, Sandra

AU - Filburn, Charles R.

AU - Sacktor, Bertram

PY - 1989/7/1

Y1 - 1989/7/1

N2 - The nervous system may play a role in regulation of bone metabolism. The effects of norepinephrine(NE), vasoactive intestinal peptide(VIP), and ATP on cytosolic Ca2+ were assessed in a rat osteoblast-like osteosarcoma cell line (UMR-106) responsive to PTH. All three transmitters transiently increased Ca2+, with ATP≫PTH>NE=VIP, and then caused sustained increases in Ca2+. The ATP-induced transient resulted from mobilization of intracellular Ca2+ store, while NE and VIP-induced transients also involved influx of Ca2+. Later sustained increases by all agonists were dependent upon extracellular Ca2+. Release of intracellular Ca2+ by ATP was associated with a marked increase in IP3 but without a significant change in cAMP. NE, VIP, and ATP, through regulation of Ca2+ metabolism, may be involved in various osteoporotic conditions.

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KW - cytosolic Ca

KW - neurotransmitter

KW - purinergic receptor

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Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells

Abstract

Keywords

ASJC Scopus subject areas

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