TY - JOUR
T1 - Neuropilin is a semaphorin III receptor
AU - Kolodkin, Alex L.
AU - Levengood, Dorothy V.
AU - Rowe, Erica G.
AU - Tai, Yu Tzu
AU - Giger, Roman J.
AU - Ginty, David D.
N1 - Funding Information:
A. L. K. and D. D. G. are senior coauthors. We thank C.-M. Fan, A. Lanahan, and S. Gillespie for invaluable advice; S. Gillespie for the immunoblot presented in Figure 1 E; J. Senft and C. Demma for DNA sequence analysis and C. Riley for protein sequence alignments; Y. Liu for help in the initial identification of Npn-2; S. Gillespie for help with figures; A. Riccio, S. Zou, and J. M. Hardwick for help with the dissociated DRG cultures; H. He and M. Tessier-Lavigne for communication of results prior to publication; R. Mains and R. Marx for help with the design and construction of myc-neuropilin ex ; C.-M. Fan, A. Ghosh, S. Gillespie, and J. Nathans for critical reading of the manuscript; and members of the Ginty and Kolodkin laboratories for helpful discussions. This work was supported by grants from The Esther A. and Joseph Klingenstein Fund, the McKnight Endowment Fund for Neuroscience, the Searle Scholars Program/The Chicago Community Trust, and the Whitehall Foundation (A. L. K.); an American Cancer Society Junior Faculty Research Award, a grant from The Esther A. and Joseph Klingenstein Fund, and a Pew Scholars Award (D. D. G.); a National Institutes of Health National Research Service Award (E. R.); and the Schweizerischen Nationalfonds (R. G.).
PY - 1997/8/22
Y1 - 1997/8/22
N2 - The semaphorin family contains a large number of phylogenetically conserved proteins and includes several members that have been shown to function in repulsive axon guidance. Semaphorin II (Sema III) is a secreted protein that in vitro causes neuronal growth cone collapse and chemorepulsion of neurites, and in vivo is required for correct sensory afferent innervation and other aspects of development. The mechanism of Sema III function, however, is unknown. Here, we report that neuropilin, a type I transmembrane protein implicated in aspects of neurodevelopment, is a Sema III receptor. We also describe the identification of neuropilin-2, a related neuropilin family member, and show that neuropilin and neuropilin-2 are expressed in overlapping, yet distinct, populations of neurons in the rat embryonic nervous system.
AB - The semaphorin family contains a large number of phylogenetically conserved proteins and includes several members that have been shown to function in repulsive axon guidance. Semaphorin II (Sema III) is a secreted protein that in vitro causes neuronal growth cone collapse and chemorepulsion of neurites, and in vivo is required for correct sensory afferent innervation and other aspects of development. The mechanism of Sema III function, however, is unknown. Here, we report that neuropilin, a type I transmembrane protein implicated in aspects of neurodevelopment, is a Sema III receptor. We also describe the identification of neuropilin-2, a related neuropilin family member, and show that neuropilin and neuropilin-2 are expressed in overlapping, yet distinct, populations of neurons in the rat embryonic nervous system.
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U2 - 10.1016/S0092-8674(00)80535-8
DO - 10.1016/S0092-8674(00)80535-8
M3 - Article
C2 - 9288754
AN - SCOPUS:0030829388
SN - 0092-8674
VL - 90
SP - 753
EP - 762
JO - Cell
JF - Cell
IS - 4
ER -