Neuropilin-1 expression by endothelial cells and retinal pigment epithelial cells in choroidal neovascular membranes

Jennifer I. Lim, Christine Spee, Masanori Hangai, Jorge Rocha, Howard S. Ying, Stephen J. Ryan, David R. Hinton

Research output: Contribution to journalArticlepeer-review

Abstract

• PURPOSE: To determine if vascular endothelial growth factor (VEGF) coreceptor neuropilin-1 (NP-1) is expressed in choroidal neovascularization (CNV) and to localize the expression. • DESIGN: Laboratory investigation. • METHODS: Six CNV membranes (CNVMs) obtained from patients with subfoveal CNV attributable to age-related macular degeneration (AMD) underwent immunohistochemistry for VEGF receptor-2 (VEGFR-2) and NP-1. The positive cell types were identified by double staining with anticytokeratin, anti-CD31, and antismooth muscle actin (SMA). • RESULTS: Immunohistochemical staining revealed positivity for VEGFR-2 and NP-1 in all six CNVMs. Both receptors were strongly expressed by new choroidal endothelial cell forming vessels and CD-31-positive cells in the nonvascular area. They were also expressed in the pigmented retinal pigment epithelial layer and by cytokeratin-positive, nonpigmented retinal pigment epithelium cells in the nonvascular area. The nonpigmented retinal pigment epithelium cells positive for VEGFR-2 and NP-1 were highly colocalized with SMA. • CONCLUSIONS: The presence of both VEGF and NP-1 suggest that NP-1 may play a role in the evolution of CNV in AMD.

Original languageEnglish (US)
Pages (from-to)1044-1050.e2
JournalAmerican journal of ophthalmology
Volume140
Issue number6
DOIs
StatePublished - Dec 2005

ASJC Scopus subject areas

  • Ophthalmology

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