TY - JOUR
T1 - Neuropeptide delivery to synapses by long-range vesicle circulation and sporadic capture
AU - Wong, Man Yan
AU - Zhou, Chaoming
AU - Shakiryanova, Dinara
AU - Lloyd, Thomas E.
AU - Deitcher, David L.
AU - Levitan, Edwin S.
N1 - Funding Information:
We thank Daniel Altschuler (University of Pittsburgh) for comments; Haig Keshishian (Yale University) for the Geneswitch flies; Eric Rulifson (UCSF) for the preproDilp2 clone; Dick Nässel (Stockholm University) for anti-proctolin precursor serum; and Yuh-Nung Jan (UCSF) for the UAS-ManII-EGFP flies. This work was supported by a grant from the National Institutes of Health (NIH R01 NS32385 to E.S.L.).
PY - 2012/3/2
Y1 - 2012/3/2
N2 - Neurotransmission requires anterograde axonal transport of dense core vesicles (DCVs) containing neuropeptides and active zone components from the soma to nerve terminals. However, it is puzzling how one-way traffic could uniformly supply sequential release sites called en passant boutons. Here, Drosophila neuropeptide-containing DCVs are tracked in vivo for minutes with a new method called simultaneous photobleaching and imaging (SPAIM). Surprisingly, anterograde DCVs typically bypass proximal boutons to accumulate initially in the most distal bouton. Then, excess distal DCVs undergo dynactin-dependent retrograde transport back through proximal boutons into the axon. Just before re-entering the soma, DCVs again reverse for another round of anterograde axonal transport. While circulating over long distances, both anterograde and retrograde DCVs are captured sporadically in en passant boutons. Therefore, vesicle circulation, which includes long-range retrograde transport and inefficient bidirectional capture, overcomes the limitations of one-way anterograde transport to uniformly supply release sites with DCVs.
AB - Neurotransmission requires anterograde axonal transport of dense core vesicles (DCVs) containing neuropeptides and active zone components from the soma to nerve terminals. However, it is puzzling how one-way traffic could uniformly supply sequential release sites called en passant boutons. Here, Drosophila neuropeptide-containing DCVs are tracked in vivo for minutes with a new method called simultaneous photobleaching and imaging (SPAIM). Surprisingly, anterograde DCVs typically bypass proximal boutons to accumulate initially in the most distal bouton. Then, excess distal DCVs undergo dynactin-dependent retrograde transport back through proximal boutons into the axon. Just before re-entering the soma, DCVs again reverse for another round of anterograde axonal transport. While circulating over long distances, both anterograde and retrograde DCVs are captured sporadically in en passant boutons. Therefore, vesicle circulation, which includes long-range retrograde transport and inefficient bidirectional capture, overcomes the limitations of one-way anterograde transport to uniformly supply release sites with DCVs.
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U2 - 10.1016/j.cell.2011.12.036
DO - 10.1016/j.cell.2011.12.036
M3 - Article
C2 - 22385966
AN - SCOPUS:84857808572
SN - 0092-8674
VL - 148
SP - 1029
EP - 1038
JO - Cell
JF - Cell
IS - 5
ER -