Neuronal pathways involved in abdominal surgery-induced gastric ileus in rats

E. Barquist, B. Bonaz, V. Martinez, J. Rivier, M. J. Zinner, Y. Taché

Research output: Contribution to journalArticle

Abstract

The 20-min rate of gastric emptying of a noncaloric solution and c-fos expression detected by immunohistochemistry in the brain were monitored 3 h after abdominal surgery performed under 10-min enflurane anesthesia in rats. Abdominal surgery (laparotomy and 1-min manipulation of the cecum) decreased gastric emptying from 60.8 ± 3.4 to 25.9 ± 3.4%. Capsaicin applied to the celiac/superior mesenteric ganglia 2 wk before the experiment reduced the delay in gastric emptying induced by abdominal surgery (46.3 ± 3.4%), whereas perivagal capsaicin application had no effect (23.6 ± 7.9%). The corticotropin-releasing factor (CRF) antagonist [D- Phe12,Nle21,38,C(α)MeLeu37]CRF-(12-41) injected intracisternally (10-20 μg) prevented postoperative gastroparesis induced by surgery, while having no effect on basal gastric emptying. Abdominal surgery increased the number of Fos-positive cells in brain nuclei regulating autonomic outflow: the nucleus of the solitary tract, locus ceruleus, paraventricular nucleus, and supraoptic nucleus of the hypothalamus. These data indicate that capsaicin-sensitive splanchnic afferent fibers and activation of CRF receptors in the brain are part of the neuronal circuitry mediating gastric stasis 3 h after abdominal surgery.

Original languageEnglish (US)
Pages (from-to)R888-R894
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume270
Issue number4 39-4
StatePublished - May 13 1996

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Keywords

  • c- fos
  • capsaicin
  • corticotropin-releasing factor
  • locus ceruleus
  • nucleus of the solitary tract
  • paraventricular nucleus of the hypothalamus
  • splanchnic afferent
  • supraoptic nucleus

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Barquist, E., Bonaz, B., Martinez, V., Rivier, J., Zinner, M. J., & Taché, Y. (1996). Neuronal pathways involved in abdominal surgery-induced gastric ileus in rats. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 270(4 39-4), R888-R894.