Neuronal nitric oxide signaling regulates erection recovery after cavernous nerve injury

Sena F. Sezen, Gwen Lagoda, Arthur Burnett

Research output: Contribution to journalArticle

Abstract

Nitric oxide is the major neuronal mediator of penile erection but its role in erectile function status after cavernous nerve injury is uncertain. We determined the function of neuronal nitric oxide signaling in the pathobiology of erectile function recovery after partial cavernous nerve injury using genetic and pharmacological mouse experimental paradigms. Erectile function was evaluated in 5 to 7 wild-type and neuronal nitric oxide synthase-α knockout mice per group 1, 3 and 7 days after unilateral crush or sham injury, at day 7 in wild-type mice treated with the nitric oxide synthase inhibitor L-NAME (l-nitro arginine methyl ester) (Sigma-Aldrich®) at baseline and for 6 days after unilateral crush injury. Apoptosis in the penis was evaluated by Western blot analysis of p-Akt-S473, 3-nitrotyrosine and caspase-3 after bilateral crush injury. Intracavernous pressure was significantly decreased at 1, 3 and 7 days in wild-type mice but only at day 1 in knockout mice after unilateral crush injury compared with sham treatment values (p

Original languageEnglish (US)
Pages (from-to)757-763
Number of pages7
JournalJournal of Urology
Volume187
Issue number2
DOIs
StatePublished - Feb 2012

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Nitric Oxide
Knockout Mice
Wounds and Injuries
Penile Erection
Nitric Oxide Synthase Type I
Recovery of Function
Penis
Nitric Oxide Synthase
Caspase 3
Western Blotting
Placebos
Pharmacology
Apoptosis
Pressure
Crush Injuries
3-nitrotyrosine
arginine methyl ester

Keywords

  • apoptosis
  • erectile dysfunction
  • nitric oxide synthase type I
  • penis
  • prostatectomy

ASJC Scopus subject areas

  • Urology

Cite this

Neuronal nitric oxide signaling regulates erection recovery after cavernous nerve injury. / Sezen, Sena F.; Lagoda, Gwen; Burnett, Arthur.

In: Journal of Urology, Vol. 187, No. 2, 02.2012, p. 757-763.

Research output: Contribution to journalArticle

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