Neuron-glia signaling in developing retina mediated by neurotransmitter spillover

Juliana M. Rosa, Rémi Bos, Georgeann S. Sack, Cécile Fortuny, Amit Agarwal, Dwight E Bergles, John G. Flannery, Marla B. Feller

Research output: Contribution to journalArticle

Abstract

Neuron-glia interactions play a critical role in the maturation of neural circuits; however, little is known about the pathways that mediate their communication in the developing CNS. We investigated neuron-glia signaling in the developing retina, where we demonstrate that retinal waves reliably induce calcium transients in Mü ller glial cells (MCs). During cholinergic waves, MC calcium transients were blocked by muscarinic acetylcholine receptor antagonists, whereas during glutamatergic waves, MC calcium transients were inhibited by ionotropic glutamate receptor antagonists, indicating that the responsiveness of MCs changes to match the neurotransmitter used to support retinal waves. Using an optical glutamate sensor we show that the decline in MC calcium transients is caused by a reduction in the amount of glutamate reaching MCs. Together, these studies indicate that neurons and MCs exhibit correlated activity during a critical period of retinal maturation that is enabled by neurotransmitter spillover from retinal synapses.

Original languageEnglish (US)
Article numbere09590
Pages (from-to)1-20
Number of pages20
JournaleLife
Volume4
Issue numberAUGUST2015
DOIs
StatePublished - Aug 14 2015

Fingerprint

Neuroglia
Neurons
Neurotransmitter Agents
Retina
Calcium
Glutamic Acid
Ionotropic Glutamate Receptors
Excitatory Amino Acid Antagonists
Optical sensors
Cholinergic Antagonists
Muscarinic Receptors
Synapses
Cholinergic Agents
Networks (circuits)
Communication

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Rosa, J. M., Bos, R., Sack, G. S., Fortuny, C., Agarwal, A., Bergles, D. E., ... Feller, M. B. (2015). Neuron-glia signaling in developing retina mediated by neurotransmitter spillover. eLife, 4(AUGUST2015), 1-20. [e09590]. https://doi.org/10.7554/eLife.09590

Neuron-glia signaling in developing retina mediated by neurotransmitter spillover. / Rosa, Juliana M.; Bos, Rémi; Sack, Georgeann S.; Fortuny, Cécile; Agarwal, Amit; Bergles, Dwight E; Flannery, John G.; Feller, Marla B.

In: eLife, Vol. 4, No. AUGUST2015, e09590, 14.08.2015, p. 1-20.

Research output: Contribution to journalArticle

Rosa, JM, Bos, R, Sack, GS, Fortuny, C, Agarwal, A, Bergles, DE, Flannery, JG & Feller, MB 2015, 'Neuron-glia signaling in developing retina mediated by neurotransmitter spillover', eLife, vol. 4, no. AUGUST2015, e09590, pp. 1-20. https://doi.org/10.7554/eLife.09590
Rosa JM, Bos R, Sack GS, Fortuny C, Agarwal A, Bergles DE et al. Neuron-glia signaling in developing retina mediated by neurotransmitter spillover. eLife. 2015 Aug 14;4(AUGUST2015):1-20. e09590. https://doi.org/10.7554/eLife.09590
Rosa, Juliana M. ; Bos, Rémi ; Sack, Georgeann S. ; Fortuny, Cécile ; Agarwal, Amit ; Bergles, Dwight E ; Flannery, John G. ; Feller, Marla B. / Neuron-glia signaling in developing retina mediated by neurotransmitter spillover. In: eLife. 2015 ; Vol. 4, No. AUGUST2015. pp. 1-20.
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