Neuromuscular junction in a microfluidic device

Hyun S ung Park; Su Liu; John McDonald; Nitish Thakor; In H ong Yang

doi:10.1109/EMBC.2013.6610130

Neuromuscular junction in a microfluidic device

Hyun S ung Park, Su Liu, John McDonald, Nitish Thakor, In H ong Yang

Research output: Contribution to journal › Article › peer-review

Abstract

Malfunctions at the site of neuromuscular junction (NMJ) of post-injuries or diseases are major barriers to recovery of function. The ability to efficiently derive motor neurons (MN) from embryonic stem cells has indicated promise toward the development of new therapies in increasing functional outcomes post injury. Recent advances in micro-technologies have provided advanced culture platforms allowing compartmentalization of sub-cellular components of neurons. In this study, we combined these advances in science and technology to develop a compartmentalized in vitro NMJ model. The developed NMJ system is between mouse embryonic stem cell (mESC)-derived MNs and c2c12 myotubes cultured in a compartmentalized polydimethylsiloxane (PDMS) microfluidic device. While some functional in vitro NMJ systems have been reported, this system would further contribute to research in NMJ-related diseases by providing a system to study the site of action of NMJ aimed at improving promoting better functional recovery.

Original language	English (US)
Pages (from-to)	2833-2835
Number of pages	3
Journal	Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
Volume	2013
DOIs	https://doi.org/10.1109/EMBC.2013.6610130
State	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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Neuromuscular junction in a microfluidic device. / Park, Hyun S ung; Liu, Su; McDonald, John et al.
In: Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, Vol. 2013, 2013, p. 2833-2835.

Research output: Contribution to journal › Article › peer-review

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abstract = "Malfunctions at the site of neuromuscular junction (NMJ) of post-injuries or diseases are major barriers to recovery of function. The ability to efficiently derive motor neurons (MN) from embryonic stem cells has indicated promise toward the development of new therapies in increasing functional outcomes post injury. Recent advances in micro-technologies have provided advanced culture platforms allowing compartmentalization of sub-cellular components of neurons. In this study, we combined these advances in science and technology to develop a compartmentalized in vitro NMJ model. The developed NMJ system is between mouse embryonic stem cell (mESC)-derived MNs and c2c12 myotubes cultured in a compartmentalized polydimethylsiloxane (PDMS) microfluidic device. While some functional in vitro NMJ systems have been reported, this system would further contribute to research in NMJ-related diseases by providing a system to study the site of action of NMJ aimed at improving promoting better functional recovery. ",

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Neuromuscular junction in a microfluidic device

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