Abstract
The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-18 |
| Number of pages | 8 |
| Journal | Advances in Experimental Medicine and Biology |
| Volume | 346 |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes. / Qu, Y.; Campbell, D. L.; Himmel, H. H.; Strauss, H. C.; De Mey, J.; Marban, E.; Prinzen, F.; Somlyo, A.; Landesberg, A.
In: Advances in Experimental Medicine and Biology, Vol. 346, 1993, p. 11-18.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes
AU - Qu, Y.
AU - Campbell, D. L.
AU - Himmel, H. H.
AU - Strauss, H. C.
AU - De Mey, J.
AU - Marban, E.
AU - Prinzen, F.
AU - Somlyo, A.
AU - Landesberg, A.
PY - 1993
Y1 - 1993
N2 - The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.
AB - The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.
UR - http://www.scopus.com/inward/record.url?scp=0027753347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027753347&partnerID=8YFLogxK
M3 - Article
C2 - 7910429
AN - SCOPUS:0027753347
VL - 346
SP - 11
EP - 18
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -