Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes

Y. Qu; D. L. Campbell; H. H. Himmel; H. C. Strauss; J. De Mey; E. Marban; F. Prinzen; A. Somlyo; A. Landesberg

Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes

Y. Qu, D. L. Campbell, H. H. Himmel, H. C. Strauss, J. De Mey, E. Marban, F. Prinzen, A. Somlyo, A. Landesberg

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of extracellular ATP on the L-type Ca²⁺ current (I(Ca), action potential, and resting and intracellular Ca²⁺ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca²⁺ levels; however, it did decrease the Ca²⁺ transient. The effects of extracellular ATP were mediated independently of adenosine A₁ receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.

Original language	English (US)
Pages (from-to)	11-18
Number of pages	8
Journal	Advances in Experimental Medicine and Biology
Volume	346
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{a43aa313cfe440579eee6b08ddc6fbec,

title = "Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes",

abstract = "The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.",

author = "Y. Qu and Campbell, {D. L.} and Himmel, {H. H.} and Strauss, {H. C.} and {De Mey}, J. and E. Marban and F. Prinzen and A. Somlyo and A. Landesberg",

year = "1993",

language = "English (US)",

volume = "346",

pages = "11--18",

journal = "Advances in Experimental Medicine and Biology",

issn = "0065-2598",

publisher = "Springer New York",

}

TY - JOUR

T1 - Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes

AU - Qu, Y.

AU - Campbell, D. L.

AU - Himmel, H. H.

AU - Strauss, H. C.

AU - De Mey, J.

AU - Marban, E.

AU - Prinzen, F.

AU - Somlyo, A.

AU - Landesberg, A.

PY - 1993

Y1 - 1993

N2 - The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.

AB - The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.

UR - http://www.scopus.com/inward/record.url?scp=0027753347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027753347&partnerID=8YFLogxK

M3 - Article

C2 - 7910429

AN - SCOPUS:0027753347

VL - 346

SP - 11

EP - 18

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -

Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this