Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes

Y. Qu, D. L. Campbell, H. H. Himmel, H. C. Strauss, J. De Mey, E. Marban, F. Prinzen, A. Somlyo, A. Landesberg

Research output: Contribution to journalArticle

Abstract

The effects of extracellular ATP on the L-type Ca2+ current (I(Ca), action potential, and resting and intracellular Ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. Extracellular ATP decreased the peak amplitude of I(Ca) in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I(Ca) was well described by a conventional Michaelis- Menten relationship with a half maximal inhibitory concentration of 1 μM and a maximal effect of 50%. Extracellular ATP did not change the resting myoplasmic Ca2+ levels; however, it did decrease the Ca2+ transient. The effects of extracellular ATP were mediated independently of adenosine A1 receptors and a pertussis-toxin sensitive G protein. Pharmacological characterization of receptor subtype using ATP analogs was consistent with ATP binding to a P(2Y) type receptor.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalAdvances in Experimental Medicine and Biology
Volume346
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Qu, Y., Campbell, D. L., Himmel, H. H., Strauss, H. C., De Mey, J., Marban, E., Prinzen, F., Somlyo, A., & Landesberg, A. (1993). Neuromodulation of calcium current by extracellular ATP in isolated ventricular myocytes. Advances in Experimental Medicine and Biology, 346, 11-18.