Neuroinflammation in primary blast neurotrauma: Time course and prevention by torso shielding

Leyan Xu, Michele Schaefer, Raleigh M. Linville, Ayushi Aggarwal, Wangui Mbuguiro, Brock A. Wester, Vassilis El Koliatsos

Research output: Contribution to journalArticle

Abstract

Mechanisms of primary blast injury caused by overpressure are not fully understood. In particular, the presence and time course of neuroinflammation are unknown and so are the signatures of reactive inflammatory cells, especially the neuroprotective versus injurious roles of microglia. In general, chronic microglial activation in the injured brain suggests a pro-degenerative role for these reactive cells. In this study, we investigated the temporal dynamics of microglial activation in the brain of mice exposed to mild-moderate blast in a shock tube. Because, in our previous work, we had found that torso shielding with rigid Plexiglas attenuates traumatic axonal injury in the brain, we also evaluated neuroinflammatory microglial responses in animals with torso protection at 7 days post blast injury. Because of the prominent involvement of the visual system in blast TBI in rodents, activated microglial cells were counted in the optic tract at various time points post-injury with stereological methods. Cell counts (activated microglial cell densities) from subjects exposed to blast TBI were compared with counts from corresponding sham animals. We found that mild-moderate blast injury causes focal activation of microglia in certain white matter tracts, including the visual pathway. In the optic tract, the density of activated microglial profiles gradually intensified from 3 to 15 days post-injury and then became attenuated at 30 days. Torso protection significantly reduced microglial activation at 7 days. These findings shed light into mechanisms of primary blast neurotrauma and may suggest novel diagnostic and monitoring methods for patients. They leave open the question of whether microglial activation post blast is protective or detrimental, although response is time limited. Finally, our findings confirm the protective role of torso shielding and stress the importance of improved or optimized body gear for warfighters or other individuals at risk for blast exposure.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalExperimental Neurology
Volume277
DOIs
StatePublished - Mar 1 2016

Fingerprint

Torso
Blast Injuries
Microglia
Cell Count
Visual Pathways
Wounds and Injuries
Brain
Physiologic Monitoring
Polymethyl Methacrylate
Brain Injuries
Rodentia
Shock
Optic Tract

Keywords

  • Microglia
  • Neurotrauma
  • Optic tract
  • Primary blast injury
  • Traumatic axonal injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Neuroinflammation in primary blast neurotrauma : Time course and prevention by torso shielding. / Xu, Leyan; Schaefer, Michele; Linville, Raleigh M.; Aggarwal, Ayushi; Mbuguiro, Wangui; Wester, Brock A.; Koliatsos, Vassilis El.

In: Experimental Neurology, Vol. 277, 01.03.2016, p. 268-274.

Research output: Contribution to journalArticle

Xu, Leyan ; Schaefer, Michele ; Linville, Raleigh M. ; Aggarwal, Ayushi ; Mbuguiro, Wangui ; Wester, Brock A. ; Koliatsos, Vassilis El. / Neuroinflammation in primary blast neurotrauma : Time course and prevention by torso shielding. In: Experimental Neurology. 2016 ; Vol. 277. pp. 268-274.
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