Neurite outgrowth in retinal ganglion cell culture.

John B. Kerrison, Donald J. Zack

Research output: Contribution to journalArticle

Abstract

Retinal ganglion cells (RGC) are the projection neurons of the eye. The RGC is the primary cell type injured in a variety of diseases of the optic nerve, including glaucoma and optic neuritis. The most well-established extrinsic signal of RGC survival and axonal outgrowth is the neurotrophin brain-derived neurotrophic factor. An immunopurification system has been adapted in order to filter large enough quantities of RGCs from the mixed population of retinal neurons in order to perform high-throughput screening in a 96-well format. Using this assay, the screening of a combinatorial chemical library for compounds with a similar effect to brain-derived neurotrophic factor may be preformed. Follow-up validation studies are performed by evaluating for a dose-response relationship.

Original languageEnglish (US)
Pages (from-to)427-434
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume356
StatePublished - 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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