Abstract
Retinal ganglion cells (RGC) are the projection neurons of the eye. The RGC is the primary cell type injured in a variety of diseases of the optic nerve, including glaucoma and optic neuritis. The most well-established extrinsic signal of RGC survival and axonal outgrowth is the neurotrophin brain-derived neurotrophic factor. An immunopurification system has been adapted in order to filter large enough quantities of RGCs from the mixed population of retinal neurons in order to perform high-throughput screening in a 96-well format. Using this assay, the screening of a combinatorial chemical library for compounds with a similar effect to brain-derived neurotrophic factor may be preformed. Follow-up validation studies are performed by evaluating for a dose-response relationship.
Original language | English (US) |
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Pages (from-to) | 427-434 |
Number of pages | 8 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 356 |
State | Published - 2007 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics