Neural Stem Cells Reduce Brain Injury After Unilateral Carotid Ligation

Anne M. Comi, Eunpi Cho, Justin D. Mulholland, Andrew Hooper, Qun Li, Yun Qu, Devin S. Gary, John W. McDonald, Michael V. Johnston

Research output: Contribution to journalArticle

Abstract

Neonatal stroke presents with seizures and results in neurologic morbidity, including epilepsy, hemiparesis, and cognitive deficits. Stem cell-based therapy offers a possible therapeutic strategy for neonatal stroke. We developed an immature mouse model of stroke with acute seizures and ischemic brain injury. Postnatal day 12 CD1 mice received right-sided carotid ligation. Two or 7 days after ligation, mice received an intrastriatal injection of B5 embryonic stem cell-derived neural stem cells. Four weeks after ligation, hemispheric brain atrophy was measured. Pups receiving stem cells 2 days after ligation had less severe hemispheric brain atrophy compared with either noninjected or vehicle-injected ligated controls. Transplanted cells survived, but 3 out of 10 pups injected with stem cells developed local tumors. No difference in hemispheric brain atrophy was seen in mice injected with stem cells 7 days after ligation. Neural stem cells have the potential to ameliorate ischemic injury in the immature brain, although tumor development is a serious concern.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalPediatric Neurology
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2008

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology

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