Abstract
Neonatal stroke presents with seizures and results in neurologic morbidity, including epilepsy, hemiparesis, and cognitive deficits. Stem cell-based therapy offers a possible therapeutic strategy for neonatal stroke. We developed an immature mouse model of stroke with acute seizures and ischemic brain injury. Postnatal day 12 CD1 mice received right-sided carotid ligation. Two or 7 days after ligation, mice received an intrastriatal injection of B5 embryonic stem cell-derived neural stem cells. Four weeks after ligation, hemispheric brain atrophy was measured. Pups receiving stem cells 2 days after ligation had less severe hemispheric brain atrophy compared with either noninjected or vehicle-injected ligated controls. Transplanted cells survived, but 3 out of 10 pups injected with stem cells developed local tumors. No difference in hemispheric brain atrophy was seen in mice injected with stem cells 7 days after ligation. Neural stem cells have the potential to ameliorate ischemic injury in the immature brain, although tumor development is a serious concern.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 86-92 |
| Number of pages | 7 |
| Journal | Pediatric Neurology |
| Volume | 38 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2008 |
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ASJC Scopus subject areas
- Clinical Neurology
- Pediatrics, Perinatology, and Child Health
- Developmental Neuroscience
- Neurology
Cite this
Neural Stem Cells Reduce Brain Injury After Unilateral Carotid Ligation. / Comi, Anne Marie Spalding; Cho, Eunpi; Mulholland, Justin D.; Hooper, Andrew; Li, Qun; Qu, Yun; Gary, Devin S.; McDonald, John W.; Johnston, Michael V.
In: Pediatric Neurology, Vol. 38, No. 2, 02.2008, p. 86-92.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neural Stem Cells Reduce Brain Injury After Unilateral Carotid Ligation
AU - Comi, Anne Marie Spalding
AU - Cho, Eunpi
AU - Mulholland, Justin D.
AU - Hooper, Andrew
AU - Li, Qun
AU - Qu, Yun
AU - Gary, Devin S.
AU - McDonald, John W.
AU - Johnston, Michael V
PY - 2008/2
Y1 - 2008/2
N2 - Neonatal stroke presents with seizures and results in neurologic morbidity, including epilepsy, hemiparesis, and cognitive deficits. Stem cell-based therapy offers a possible therapeutic strategy for neonatal stroke. We developed an immature mouse model of stroke with acute seizures and ischemic brain injury. Postnatal day 12 CD1 mice received right-sided carotid ligation. Two or 7 days after ligation, mice received an intrastriatal injection of B5 embryonic stem cell-derived neural stem cells. Four weeks after ligation, hemispheric brain atrophy was measured. Pups receiving stem cells 2 days after ligation had less severe hemispheric brain atrophy compared with either noninjected or vehicle-injected ligated controls. Transplanted cells survived, but 3 out of 10 pups injected with stem cells developed local tumors. No difference in hemispheric brain atrophy was seen in mice injected with stem cells 7 days after ligation. Neural stem cells have the potential to ameliorate ischemic injury in the immature brain, although tumor development is a serious concern.
AB - Neonatal stroke presents with seizures and results in neurologic morbidity, including epilepsy, hemiparesis, and cognitive deficits. Stem cell-based therapy offers a possible therapeutic strategy for neonatal stroke. We developed an immature mouse model of stroke with acute seizures and ischemic brain injury. Postnatal day 12 CD1 mice received right-sided carotid ligation. Two or 7 days after ligation, mice received an intrastriatal injection of B5 embryonic stem cell-derived neural stem cells. Four weeks after ligation, hemispheric brain atrophy was measured. Pups receiving stem cells 2 days after ligation had less severe hemispheric brain atrophy compared with either noninjected or vehicle-injected ligated controls. Transplanted cells survived, but 3 out of 10 pups injected with stem cells developed local tumors. No difference in hemispheric brain atrophy was seen in mice injected with stem cells 7 days after ligation. Neural stem cells have the potential to ameliorate ischemic injury in the immature brain, although tumor development is a serious concern.
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UR - http://www.scopus.com/inward/citedby.url?scp=38149091867&partnerID=8YFLogxK
U2 - 10.1016/j.pediatrneurol.2007.10.007
DO - 10.1016/j.pediatrneurol.2007.10.007
M3 - Article
C2 - 18206788
AN - SCOPUS:38149091867
VL - 38
SP - 86
EP - 92
JO - Pediatric Neurology
JF - Pediatric Neurology
SN - 0887-8994
IS - 2
ER -