Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons

Hong Jun Song, Charles F. Stevens, Fred H. Gage

Research output: Contribution to journalArticle

Abstract

Neural stem cells are present both in the developing nervous system and in the adult nervous system of all mammals, including humans. Little is known, however, about the extent to which stem cells in adults can give rise to new neurons. We used immunocytochemistry, electron microscopy, fluorescence microscopy (FM imaging) and electrophysiology to demonstrate that progeny of adult rat neural stem cells, when co-cultured with primary neurons and astrocytes from neonatal hippocampus, develop into electrically active neurons and integrate into neuronal networks with functional synaptic transmission. We also found that functional neurogenesis from adult stem cells is possible in co-culture with astrocytes from neonatal and adult hippocampus. These studies show that neural stem cells derived from adult tissues, like those derived from embryonic tissues, retain the potential to differentiate into functional neurons with essential properties of mature CNS neurons.

Original languageEnglish (US)
Pages (from-to)438-445
Number of pages8
JournalNature Neuroscience
Volume5
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

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Neural Stem Cells
Hippocampus
Neurons
Adult Stem Cells
Astrocytes
Nervous System
Electrophysiology
Neurogenesis
Coculture Techniques
Fluorescence Microscopy
Synaptic Transmission
Mammals
Electron Microscopy
Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons. / Song, Hong Jun; Stevens, Charles F.; Gage, Fred H.

In: Nature Neuroscience, Vol. 5, No. 5, 2002, p. 438-445.

Research output: Contribution to journalArticle

Song, Hong Jun ; Stevens, Charles F. ; Gage, Fred H. / Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons. In: Nature Neuroscience. 2002 ; Vol. 5, No. 5. pp. 438-445.
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