Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C

Seth D. Crosby, Rosalie Ann Veile, Helen Donis-Keller, Jay M Baraban, Ratan V. Bhat, Kelli S. Simburger, Jeffrey Milbrandt

Research output: Contribution to journalArticle

Abstract

The nerve growth factor-induced clone C (NGFI-C) gene encodes a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains; in addition, analysis of the non-zinc-finger region revealed that they share an additional five highly homologous subdomains, four of which are within the ammo terminus. The 5′ flanking region of NGFI-C contains several cAMP response elements but does not contain any serum-response elements or CArG boxes [CC(A/T)6GG], cis-acting elements commonly involved in early response gene regulation. NGFI-C mRNA was detected in neural tissues of postnatal animals, but no expression was found in rat embryos. In situ hybridization demonstrated that NGFI-C is rapidly induced in the dentate gyrus of the hippocampus after seizure, but in contrast to NGFI-A, increases in NGFI-C mRNA were not detected in the overlying cortex. By using fluorescence in situ hybridization, NGFI-C was localized to human chromosome 2p13. This region contains a constitutive fragile site that is associated with chromosomal break-points and translocations characteristic of some chronic lymphocytic leukemias.

Original languageEnglish (US)
Pages (from-to)4739-4743
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number10
StatePublished - 1992

Fingerprint

Zinc Fingers
Nerve Growth Factor
Transcription Factors
Clone Cells
Genes
Seizures
Serum Response Element
Immediate-Early Proteins
Chromosome Breakage
Parahippocampal Gyrus
Genetic Translocation
Messenger RNA
5' Flanking Region
PC12 Cells
Dentate Gyrus
Human Chromosomes
Response Elements
Pheochromocytoma
B-Cell Chronic Lymphocytic Leukemia
Fluorescence In Situ Hybridization

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C. / Crosby, Seth D.; Ann Veile, Rosalie; Donis-Keller, Helen; Baraban, Jay M; Bhat, Ratan V.; Simburger, Kelli S.; Milbrandt, Jeffrey.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 10, 1992, p. 4739-4743.

Research output: Contribution to journalArticle

Crosby, Seth D. ; Ann Veile, Rosalie ; Donis-Keller, Helen ; Baraban, Jay M ; Bhat, Ratan V. ; Simburger, Kelli S. ; Milbrandt, Jeffrey. / Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 10. pp. 4739-4743.
@article{c009a9fe9d4246cf9b9aee788d57d5af,
title = "Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C",
abstract = "The nerve growth factor-induced clone C (NGFI-C) gene encodes a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains; in addition, analysis of the non-zinc-finger region revealed that they share an additional five highly homologous subdomains, four of which are within the ammo terminus. The 5′ flanking region of NGFI-C contains several cAMP response elements but does not contain any serum-response elements or CArG boxes [CC(A/T)6GG], cis-acting elements commonly involved in early response gene regulation. NGFI-C mRNA was detected in neural tissues of postnatal animals, but no expression was found in rat embryos. In situ hybridization demonstrated that NGFI-C is rapidly induced in the dentate gyrus of the hippocampus after seizure, but in contrast to NGFI-A, increases in NGFI-C mRNA were not detected in the overlying cortex. By using fluorescence in situ hybridization, NGFI-C was localized to human chromosome 2p13. This region contains a constitutive fragile site that is associated with chromosomal break-points and translocations characteristic of some chronic lymphocytic leukemias.",
author = "Crosby, {Seth D.} and {Ann Veile}, Rosalie and Helen Donis-Keller and Baraban, {Jay M} and Bhat, {Ratan V.} and Simburger, {Kelli S.} and Jeffrey Milbrandt",
year = "1992",
language = "English (US)",
volume = "89",
pages = "4739--4743",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C

AU - Crosby, Seth D.

AU - Ann Veile, Rosalie

AU - Donis-Keller, Helen

AU - Baraban, Jay M

AU - Bhat, Ratan V.

AU - Simburger, Kelli S.

AU - Milbrandt, Jeffrey

PY - 1992

Y1 - 1992

N2 - The nerve growth factor-induced clone C (NGFI-C) gene encodes a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains; in addition, analysis of the non-zinc-finger region revealed that they share an additional five highly homologous subdomains, four of which are within the ammo terminus. The 5′ flanking region of NGFI-C contains several cAMP response elements but does not contain any serum-response elements or CArG boxes [CC(A/T)6GG], cis-acting elements commonly involved in early response gene regulation. NGFI-C mRNA was detected in neural tissues of postnatal animals, but no expression was found in rat embryos. In situ hybridization demonstrated that NGFI-C is rapidly induced in the dentate gyrus of the hippocampus after seizure, but in contrast to NGFI-A, increases in NGFI-C mRNA were not detected in the overlying cortex. By using fluorescence in situ hybridization, NGFI-C was localized to human chromosome 2p13. This region contains a constitutive fragile site that is associated with chromosomal break-points and translocations characteristic of some chronic lymphocytic leukemias.

AB - The nerve growth factor-induced clone C (NGFI-C) gene encodes a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains; in addition, analysis of the non-zinc-finger region revealed that they share an additional five highly homologous subdomains, four of which are within the ammo terminus. The 5′ flanking region of NGFI-C contains several cAMP response elements but does not contain any serum-response elements or CArG boxes [CC(A/T)6GG], cis-acting elements commonly involved in early response gene regulation. NGFI-C mRNA was detected in neural tissues of postnatal animals, but no expression was found in rat embryos. In situ hybridization demonstrated that NGFI-C is rapidly induced in the dentate gyrus of the hippocampus after seizure, but in contrast to NGFI-A, increases in NGFI-C mRNA were not detected in the overlying cortex. By using fluorescence in situ hybridization, NGFI-C was localized to human chromosome 2p13. This region contains a constitutive fragile site that is associated with chromosomal break-points and translocations characteristic of some chronic lymphocytic leukemias.

UR - http://www.scopus.com/inward/record.url?scp=0026579371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026579371&partnerID=8YFLogxK

M3 - Article

C2 - 1584812

AN - SCOPUS:0026579371

VL - 89

SP - 4739

EP - 4743

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -