Neural progenitor cells grown on hydrogel surfaces respond to the product of the transgene of encapsulated genetically engineered fibroblasts

Mihir S. Shanbhag, Justin D. Lathia, Mohamed R. Mughal, Nicola L. Francis, Nicholas Pashos, Mark P. Mattson, Margaret A. Wheatley

Research output: Contribution to journalArticlepeer-review

Abstract

Engineered tissue strategies for central nervous system (CNS) repair have the potential for localizing treatment using a wide variety of cells or growth factors. However, these strategies are often limited by their ability to address only one aspect of the injury. Here we report the Development of a novel alginate construct that acts as a multifunctional tissue scaffold for CNS repair, and as a localized growth factor Delivery vehicle. We show that the surface of this alginate construct acts as an optimal growth environment for neural progenitor cell (NPC) attachment, survival, migration, and differentiation. Importantly, we show that tailor-made alginate constructs containing brain-derived neurotrophic factor or neurotrophin-3 differentially direct lineage fates of NPCs and may therefore be useful in treating a wide variety of injuries. It is this potential for directed differentiation of a scaffold prior to implantation at the injury site that we explore here.

Original languageEnglish (US)
Pages (from-to)2936-2943
Number of pages8
JournalBiomacromolecules
Volume11
Issue number11
DOIs
StatePublished - Nov 8 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Medicine(all)

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