@article{bf8323e1bbaa44c7ac08c862b7ea4380,
title = "Neural induction of porcine-induced pluripotent stem cells and further differentiation using glioblastoma-cultured medium",
abstract = "Prior to transplantation, preclinical study of safety and efficacy of neural progenitor cells (NPCs) is needed. Therefore, it is important to generate an efficient in vitro platform for neural cell differentiation in large animal models such as pigs. In this study, porcine-induced pluripotent stem cells (iPSCs) were seeded at high cell density to a neural induction medium containing the dual Sma- and Mad-related protein (SMAD) inhibitors, a TGF-β inhibitor and BMP4 inhibitor. The dSMADi-derived NPCs showed NPC markers such as PLAG1, NESTIN and VIMENTIN and higher mRNA expression of Sox1 compared to the control. The mRNA expression of HOXB4 was found to significantly increase in the retinoic acid-treated group. NPCs propagated in vitro and generated neurospheres that are capable of further differentiation in neurons and glial cells. Gliobalstoma-cultured medium including injury-related cytokines treated porcine iPSC-NPCs survive well in vitro and showed more neuronal marker expression compared to standard control medium. Collectively, the present study developed an efficient method for production of neural commitment of porcine iPSCs into NPCs.",
keywords = "glioblastoma, induced pluripotent stem cells, neural progenitor cells, pig",
author = "Eunhye Kim and Mirae Kim and Hwang, {Seon Ung} and Jongpil Kim and Gabsang Lee and Park, {Young Seok} and Hyun, {Sang Hwan}",
note = "Funding Information: IPET through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA), Award/Award Number: 115103-02; National Research Foundation of Korea Award funded by the Korean Government, Award/Award Number: NRF-2016R1D1A1B03933191, NRF- 2017R1A2B4002546; Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration, Award/Award Number: 2017020681010101; The Global Research Funding Information: This work was supported, in part, by a grant from the “National Research Foundation of Korea Award funded by the Korean Government (NRF‐2016R1D1A1B03933191, NRF‐2017R1A2B4002546)“, “Korea Institute of Planning and Evaluation for Technology in Food, Funding Information: and Development Center (GRDC) Program through the NRF funded by the Ministry of Education in Korea, Award/Award Number: 2017K1A4A3014959 Funding Information: Agriculture, Forestry and Fisheries (IPET) through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Award No. 115103‐02)”, “The Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2017K1A4A3014959)” and “Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2017 (Award No. 2017020681010101)”, Republic of Korea. Publisher Copyright: {\textcopyright} 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.",
year = "2019",
month = mar,
doi = "10.1111/jcmm.14111",
language = "English (US)",
volume = "23",
pages = "2052--2063",
journal = "Journal of Cellular and Molecular Medicine",
issn = "1582-1838",
publisher = "Wiley-Blackwell",
number = "3",
}