Nested genetic bit analysis (N-GBA) for mutation detection in the p53 tumor suppressor gene

Steven R. Head, Yu Hui Rogers, Kalpana Parikh, Guang Lan, Stephen Anderson, Philip Goelet, Michael T. Boyce-Jacino

Research output: Contribution to journalArticlepeer-review

Abstract

There is a growing and significant demand for reliable, simple and sensitive methods for repeated scanning of a given gene or gene fragment for detection and characterization of mutations. Solid-phase sequencing by single base primer extension of nested GBA® primers on miniaturized DNA arrays can be used to effectively scan targeted sequences for missense, insertion and deletion mutations. This paper describes the use of N-GBA arrays designed to scan the sequence of a 33 base region of exon 8 of the p53 gene (codons 272-282) encompassing a hot spot for mutations associated with the development of cancer. Synthetic DNA templates containing various missense, insertion and deletion mutations, as well as DNA prepared from pancreatic and biliary tumor cells, were genotyped using the exon 8 arrays.

Original languageEnglish (US)
Pages (from-to)5065-5071
Number of pages7
JournalNucleic acids research
Volume25
Issue number24
DOIs
StatePublished - Dec 15 1997
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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