TY - JOUR
T1 - Nested genetic bit analysis (N-GBA) for mutation detection in the p53 tumor suppressor gene
AU - Head, Steven R.
AU - Rogers, Yu Hui
AU - Parikh, Kalpana
AU - Lan, Guang
AU - Anderson, Stephen
AU - Goelet, Philip
AU - Boyce-Jacino, Michael T.
PY - 1997/12/15
Y1 - 1997/12/15
N2 - There is a growing and significant demand for reliable, simple and sensitive methods for repeated scanning of a given gene or gene fragment for detection and characterization of mutations. Solid-phase sequencing by single base primer extension of nested GBA® primers on miniaturized DNA arrays can be used to effectively scan targeted sequences for missense, insertion and deletion mutations. This paper describes the use of N-GBA arrays designed to scan the sequence of a 33 base region of exon 8 of the p53 gene (codons 272-282) encompassing a hot spot for mutations associated with the development of cancer. Synthetic DNA templates containing various missense, insertion and deletion mutations, as well as DNA prepared from pancreatic and biliary tumor cells, were genotyped using the exon 8 arrays.
AB - There is a growing and significant demand for reliable, simple and sensitive methods for repeated scanning of a given gene or gene fragment for detection and characterization of mutations. Solid-phase sequencing by single base primer extension of nested GBA® primers on miniaturized DNA arrays can be used to effectively scan targeted sequences for missense, insertion and deletion mutations. This paper describes the use of N-GBA arrays designed to scan the sequence of a 33 base region of exon 8 of the p53 gene (codons 272-282) encompassing a hot spot for mutations associated with the development of cancer. Synthetic DNA templates containing various missense, insertion and deletion mutations, as well as DNA prepared from pancreatic and biliary tumor cells, were genotyped using the exon 8 arrays.
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U2 - 10.1093/nar/25.24.5065
DO - 10.1093/nar/25.24.5065
M3 - Article
C2 - 9396817
AN - SCOPUS:0031574230
SN - 0305-1048
VL - 25
SP - 5065
EP - 5071
JO - Nucleic acids research
JF - Nucleic acids research
IS - 24
ER -