Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I

Barbara Sollner-Webb, Jo Anne Kaye Wilkinson, Judith Roan, Ronald H. Reeder

Research output: Contribution to journalArticle

Abstract

The DNA regions required for accurate initiation by RNA polymerase I on the ribosomal RNA genes of Xenopus laevis consist of at least two domains within a 150 bp segment adjoining the initiation site. A region of only 13 nucleotides (from -7 to +6) appears sufficient to specify accurate and efficient initiation when various 5′ and 3′ deletion mutants are injected into oocyte nuclei. In contrast, a 10-fold larger region (from -142 to +6) is required for maximal synthesis when deletion mutants are assayed in oocyte nuclear homogenates; only a low level of synthesis is specified by the 13 bp promoter domain in this in vitro system. Site-specific mutants demonstrate that a sequence adjoining nucleotide -75 is also essential for efficient in vitro initiation. Under other conditions, transcription can be strongly affected by sequences that extend several kb upstream in the rDNA spacer and contain duplications of sequences found within the 150 bp promoter. We propose a model in which transcription of the X. laevis rRNA genes is regulated by interaction of at least three sequence domains that extend over a several kb region.

Original languageEnglish (US)
Pages (from-to)199-206
Number of pages8
JournalCell
Volume35
Issue number1
DOIs
StatePublished - 1983

Fingerprint

RNA Polymerase I
Ribosomal RNA
Xenopus laevis
Transcription
Xenopus
rRNA Genes
Oocytes
Nucleotides
Genes
Ribosomal DNA
DNA
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Sollner-Webb, B., Wilkinson, J. A. K., Roan, J., & Reeder, R. H. (1983). Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I. Cell, 35(1), 199-206. https://doi.org/10.1016/0092-8674(83)90222-2

Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I. / Sollner-Webb, Barbara; Wilkinson, Jo Anne Kaye; Roan, Judith; Reeder, Ronald H.

In: Cell, Vol. 35, No. 1, 1983, p. 199-206.

Research output: Contribution to journalArticle

Sollner-Webb, B, Wilkinson, JAK, Roan, J & Reeder, RH 1983, 'Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I', Cell, vol. 35, no. 1, pp. 199-206. https://doi.org/10.1016/0092-8674(83)90222-2
Sollner-Webb, Barbara ; Wilkinson, Jo Anne Kaye ; Roan, Judith ; Reeder, Ronald H. / Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I. In: Cell. 1983 ; Vol. 35, No. 1. pp. 199-206.
@article{74b318ccc2c647708a0f3184c9aa486d,
title = "Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I",
abstract = "The DNA regions required for accurate initiation by RNA polymerase I on the ribosomal RNA genes of Xenopus laevis consist of at least two domains within a 150 bp segment adjoining the initiation site. A region of only 13 nucleotides (from -7 to +6) appears sufficient to specify accurate and efficient initiation when various 5′ and 3′ deletion mutants are injected into oocyte nuclei. In contrast, a 10-fold larger region (from -142 to +6) is required for maximal synthesis when deletion mutants are assayed in oocyte nuclear homogenates; only a low level of synthesis is specified by the 13 bp promoter domain in this in vitro system. Site-specific mutants demonstrate that a sequence adjoining nucleotide -75 is also essential for efficient in vitro initiation. Under other conditions, transcription can be strongly affected by sequences that extend several kb upstream in the rDNA spacer and contain duplications of sequences found within the 150 bp promoter. We propose a model in which transcription of the X. laevis rRNA genes is regulated by interaction of at least three sequence domains that extend over a several kb region.",
author = "Barbara Sollner-Webb and Wilkinson, {Jo Anne Kaye} and Judith Roan and Reeder, {Ronald H.}",
year = "1983",
doi = "10.1016/0092-8674(83)90222-2",
language = "English (US)",
volume = "35",
pages = "199--206",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I

AU - Sollner-Webb, Barbara

AU - Wilkinson, Jo Anne Kaye

AU - Roan, Judith

AU - Reeder, Ronald H.

PY - 1983

Y1 - 1983

N2 - The DNA regions required for accurate initiation by RNA polymerase I on the ribosomal RNA genes of Xenopus laevis consist of at least two domains within a 150 bp segment adjoining the initiation site. A region of only 13 nucleotides (from -7 to +6) appears sufficient to specify accurate and efficient initiation when various 5′ and 3′ deletion mutants are injected into oocyte nuclei. In contrast, a 10-fold larger region (from -142 to +6) is required for maximal synthesis when deletion mutants are assayed in oocyte nuclear homogenates; only a low level of synthesis is specified by the 13 bp promoter domain in this in vitro system. Site-specific mutants demonstrate that a sequence adjoining nucleotide -75 is also essential for efficient in vitro initiation. Under other conditions, transcription can be strongly affected by sequences that extend several kb upstream in the rDNA spacer and contain duplications of sequences found within the 150 bp promoter. We propose a model in which transcription of the X. laevis rRNA genes is regulated by interaction of at least three sequence domains that extend over a several kb region.

AB - The DNA regions required for accurate initiation by RNA polymerase I on the ribosomal RNA genes of Xenopus laevis consist of at least two domains within a 150 bp segment adjoining the initiation site. A region of only 13 nucleotides (from -7 to +6) appears sufficient to specify accurate and efficient initiation when various 5′ and 3′ deletion mutants are injected into oocyte nuclei. In contrast, a 10-fold larger region (from -142 to +6) is required for maximal synthesis when deletion mutants are assayed in oocyte nuclear homogenates; only a low level of synthesis is specified by the 13 bp promoter domain in this in vitro system. Site-specific mutants demonstrate that a sequence adjoining nucleotide -75 is also essential for efficient in vitro initiation. Under other conditions, transcription can be strongly affected by sequences that extend several kb upstream in the rDNA spacer and contain duplications of sequences found within the 150 bp promoter. We propose a model in which transcription of the X. laevis rRNA genes is regulated by interaction of at least three sequence domains that extend over a several kb region.

UR - http://www.scopus.com/inward/record.url?scp=0021041865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021041865&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(83)90222-2

DO - 10.1016/0092-8674(83)90222-2

M3 - Article

C2 - 6684995

AN - SCOPUS:0021041865

VL - 35

SP - 199

EP - 206

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -