Nephrotoxicity in rats undergoing a one-hour exposure to Compound A

L. Kandel, M. J. Laster, E. I. Eger, R. L. Kerschmann, J. Martin

Research output: Contribution to journalArticle

Abstract

We previously demonstrated that rats experienced renal injury when exposed for 3-12 h to 50 ppm or more of a vinyl ether called Compound A [CF2 = C(CF3)OCH2F], a compound produced by CO2 absorbents acting on sevoflurane. These durations of exposure exceed the average duration of clinical anesthesia. We now report the effect of a 1-h exposure to 0, 100, 150, 200, 400, 600, or 800 ppm of Compound A in oxygen in 145 Wistar rats. Twenty-four hours after exposure, we obtained kidney and liver specimens for microscopic examination, applying hematoxylin and eosin, and (separately) an immunochemical marker (PCNA) for cell proliferation (regeneration). Compared with results from control rats (those breathing oxygen for 1 h), renal injury (defined as necrosis of the outer strip of the outer medullary layer or 'corticomedullary junction necrosis') occurred at and above 200 ppm. Exposure to 150 ppm produced cell regeneration (i.e., stimulated cell proliferation). We conclude that the threshold concentrations for nephrotoxicity (i.e., minimal toxicity) for a 1-h exposure to Compound A exceed the maximum concentrations (particularly those at low inflow rates) reported in clinical practice by a factor of 2-3. If these threshold effects in rats apply to humans, one 1-h exposure to sevoflurane probably would not alter usual measures of renal function.

Original languageEnglish (US)
Pages (from-to)559-563
Number of pages5
JournalAnesthesia and analgesia
Volume81
Issue number3
DOIs
StatePublished - Sep 18 1995

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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