Neospora caninum recruits host cell structures to its parasitophorous vacuole and salvages lipids from organelles

Sabrina J. Nolan, Julia Debra Romano, Thomas Luechtefeld, Isabelle Coppens

Research output: Contribution to journalArticle

Abstract

Toxoplasma gondii and Neospora caninum, which cause the diseases toxoplasmosis and neosporosis, respectively, are two closely related apicomplexan parasites. They have similar heteroxenous life cycles and conserved genomes and share many metabolic features. Despite these similarities, T. gondii and N. caninum differ in their transmission strategies and zoonotic potential. Comparative analyses of the two parasites are important to identify the unique biological features that underlie the basis of host preference and pathogenicity. T. gondii and N. caninum are obligate intravacuolar parasites; in contrast to T. gondii, events that occur during N. caninum infection remain largely uncharacterized. We examined the capability of N. caninum (Liverpool isolate) to interact with host organelles and scavenge nutrients in comparison to that of T. gondii (RH strain). N. caninum reorganizes the host microtubular cytoskeleton and attracts endoplasmic reticulum (ER), mitochondria, lysosomes, multivesicular bodies, and Golgi vesicles to its vacuole though with some notable differences from T. gondii. For example, the host ER gathers around the N. caninum parasitophorous vacuole (PV) but does not physically associate with the vacuolar membrane; the host Golgi apparatus surrounds the N. caninum PV but does not fragment into ministacks. N. caninum relies on plasma lipoproteins and scavenges cholesterol from NPC1-containing endocytic organelles. This parasite salvages sphingolipids from host Golgi Rab14 vesicles that it sequesters into its vacuole. Our data highlight a remarkable degree of conservation in the intracellular infection program of N. caninum and T. gondii. The minor differences between the two parasites related to the recruitment and rearrangement of host organelles around their vacuoles likely reflect divergent evolutionary paths.

Original languageEnglish (US)
Pages (from-to)454-473
Number of pages20
JournalEukaryotic Cell
Volume14
Issue number5
DOIs
StatePublished - 2015

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Neospora
Vacuoles
Organelles
Toxoplasma
Lipids
Parasites
Endoplasmic Reticulum
Multivesicular Bodies
Sphingolipids
Toxoplasmosis
Zoonoses
Golgi Apparatus
Lysosomes
Infection
Life Cycle Stages
Cytoskeleton
Virulence
Mitochondria
Genome

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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Neospora caninum recruits host cell structures to its parasitophorous vacuole and salvages lipids from organelles. / Nolan, Sabrina J.; Romano, Julia Debra; Luechtefeld, Thomas; Coppens, Isabelle.

In: Eukaryotic Cell, Vol. 14, No. 5, 2015, p. 454-473.

Research output: Contribution to journalArticle

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