TY - JOUR
T1 - Neoplastic transformation of RK3E by mutant β-catenin requires deregulation of Tcf/Lef transcription but not activation of c-myc expression
AU - Kolligs, Frank T.
AU - Hu, Gang
AU - Dang, Chi V.
AU - Fearon, Eric R.
PY - 1999/8
Y1 - 1999/8
N2 - Current models predict that β-catenin (β-cat) functions in Wnt signaling via activation of Tcf/Lef target genes and that its abundance is regulated by the adenomatous polyposis coli (APC) and glycogen synthase kinase 3β (GSK3β) proteins. In colon and other cancers, mutations in APC or presumptive GSK3β phosphorylation sites of β-cat are associated with constitutive activation of Tcf/Lef transcription. In spite of assumptions about its oncogenic potential, prior efforts to demonstrate that mutated β- cat will induce neoplastic transformation have yielded equivocal results. We report here that mutated, but not wild-type, β-cat proteins induced neoplastic transformation of RK3E, an adenovirus E1A-immortalized epithelial cell line. Analysis of the properties of mutant β-cat proteins and studies with a dominant negative Tcf-4 mutant indicated that the ability of β-cat to bind and activate Tcf/Lef factors is crucial for transformation, c-myc has recently been implicated as a critical Tcf-regulated target gene. However, c- myc was not consistently activated in β-cat-transformed RK3E cells, and a dominant negative c-Myc mutant protein failed to inhibit β-cat transformation. Our findings underscore the role of β-cat mutations and Tcf/Lef activation in cancer and illustrate a useful system for defining critical factors in β-cat transformation.
AB - Current models predict that β-catenin (β-cat) functions in Wnt signaling via activation of Tcf/Lef target genes and that its abundance is regulated by the adenomatous polyposis coli (APC) and glycogen synthase kinase 3β (GSK3β) proteins. In colon and other cancers, mutations in APC or presumptive GSK3β phosphorylation sites of β-cat are associated with constitutive activation of Tcf/Lef transcription. In spite of assumptions about its oncogenic potential, prior efforts to demonstrate that mutated β- cat will induce neoplastic transformation have yielded equivocal results. We report here that mutated, but not wild-type, β-cat proteins induced neoplastic transformation of RK3E, an adenovirus E1A-immortalized epithelial cell line. Analysis of the properties of mutant β-cat proteins and studies with a dominant negative Tcf-4 mutant indicated that the ability of β-cat to bind and activate Tcf/Lef factors is crucial for transformation, c-myc has recently been implicated as a critical Tcf-regulated target gene. However, c- myc was not consistently activated in β-cat-transformed RK3E cells, and a dominant negative c-Myc mutant protein failed to inhibit β-cat transformation. Our findings underscore the role of β-cat mutations and Tcf/Lef activation in cancer and illustrate a useful system for defining critical factors in β-cat transformation.
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U2 - 10.1128/mcb.19.8.5696
DO - 10.1128/mcb.19.8.5696
M3 - Article
C2 - 10409758
AN - SCOPUS:0032801892
SN - 0270-7306
VL - 19
SP - 5696
EP - 5706
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 8
ER -