Neonatal sex steroids affect responses to Seoul virus infection in male but not female Norway rats

Sabra L. Klein, Aimee L. Marson, Alan L. Scott, Gary Ketner, Gregory E. Glass

Research output: Contribution to journalArticlepeer-review


Previous studies illustrate that after inoculation with Seoul virus (i.e., the naturally occurring hantavirus found in Norway rats), adult male rats produce higher antibody responses, exhibit higher Th1 responses (i.e., IgG2a, IL-2, and IFNγ), and shed virus longer than females, but these difference are not altered by manipulation of sex steroids in adulthood. To determine whether sex steroid hormones organize adult responses to hantavirus infection, at 2-4 days of age, male rats were gonadectomized and female rats were injected with testosterone. As adults, animals were inoculated with 104 pfu of Seoul virus. Neonatally gonadectomized males (NGM), control females (CF), and neonatal testosterone-treated females (NTF) had lower anti-Seoul virus IgG and IgG2a responses than control males (CM) 20, 30, and 40 days post-inoculation. Neonatal testosterone treatment had no effect on female antibody responses to infection. NGM, CF, and NTF shed virus in saliva and feces for a shorter duration than CM. There was no effect of neonatal hormone manipulation either on the percentage of animals with detectable virus or on the number of virus copies within each target organ. Genetic males, regardless of hormone manipulation, had higher virus replication in lung tissue than did genetic females. Neonatal sex steroids influence the sexual dimorphism in host immune function but do not modify virus replication in target tissues.

Original languageEnglish (US)
Pages (from-to)736-746
Number of pages11
JournalBrain, Behavior, and Immunity
Issue number6
StatePublished - 2002


  • Activational
  • Estradiol
  • Hantavirus
  • Organizational
  • Real-time RT-PCR
  • Sex difference
  • Testosterone

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience


Dive into the research topics of 'Neonatal sex steroids affect responses to Seoul virus infection in male but not female Norway rats'. Together they form a unique fingerprint.

Cite this