Nef and LTR Sequence Variation from Sequentially Derived Human Immunodeficiency Virus Type 1 Isolates

Terry McNearney, Zuzana Hornickova, Alan Templeton, Anahid Birdwell, Richard Bryan Markham, Richard Markham, Alfred Saah, Lee Ratner

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Abstract

Variation in HIV-1 nef and LTR DNA sequences was assessed longitudinally during disease progression in four HIV-1infected subjects. Point mutations were found among quasispecies obtained at a single time point in each individual, with increasing diversity with disease progression in two of three patients for whom sufficient data were available for analysis. Deletions and rearrangements were more common in late than early stages of disease. Continued sequence evolution in HIV-1 quasispecies with nef deletions along with coexistence of nef-bearing quasispecies suggest that nef-deleted quasispecies are capable of replication in vivo, possibly complemented by quasispecies lacking such deletions and/or by adaptation to a specialized niche within the patients.

Original languageEnglish (US)
Pages (from-to)388-398
Number of pages11
JournalVirology
Volume208
Issue number1
DOIs
Publication statusPublished - Apr 1 1995

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

McNearney, T., Hornickova, Z., Templeton, A., Birdwell, A., Markham, R. B., Markham, R., ... Ratner, L. (1995). Nef and LTR Sequence Variation from Sequentially Derived Human Immunodeficiency Virus Type 1 Isolates. Virology, 208(1), 388-398. https://doi.org/10.1006/viro.1995.1166