Necrotizing enterocolitis: Intraluminal biochemistry in human neonates and a rabbit model

David A. Clark; Jeffrey E. Thompson; Leonard B. Weiner; Julia A. McMillan; Albert J. Schneider; John E. Rokahr

doi:10.1203/00006450-198509000-00010

Necrotizing enterocolitis: Intraluminal biochemistry in human neonates and a rabbit model

David A. Clark, Jeffrey E. Thompson, Leonard B. Weiner, Julia A. McMillan, Albert J. Schneider, John E. Rokahr

Research output: Contribution to journal › Article › peer-review

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Abstract

The intestinal contents of 17 neonates with necrotizing enterocolitis were analyzed for pH, carbohydrate, protein, and bacteria. The intraluminal pH was <5.0 (16/17). Sufficient carbohydrate and bacteria capable of fermenting the carbohydrate to organic acids were found. The intraluminal protein content was >5 g/dl. The variables of acid and protein were then examined in a rabbit intestinal loop model. The hemorrhagic response in individual loops was measured using Cr⁵¹ tagged red blood cells such that the microliters of blood per centimeter intestine could be determined. Loops with organic acid and protein had significantly (p < 0.01) more intramural blood than control loops. Organic acid (possibly generated by bacterial mixed acid fermentation of carbohydrate) in the presence of protein promotes intramural hemorrhage similar to that seen in neonates with necrotizing enterocolitis.

Original language	English (US)
Pages (from-to)	919-921
Number of pages	3
Journal	Pediatric research
Volume	19
Issue number	9
DOIs	https://doi.org/10.1203/00006450-198509000-00010
State	Published - Sep 1985
Externally published	Yes

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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10.1203/00006450-198509000-00010

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title = "Necrotizing enterocolitis: Intraluminal biochemistry in human neonates and a rabbit model",

abstract = "The intestinal contents of 17 neonates with necrotizing enterocolitis were analyzed for pH, carbohydrate, protein, and bacteria. The intraluminal pH was <5.0 (16/17). Sufficient carbohydrate and bacteria capable of fermenting the carbohydrate to organic acids were found. The intraluminal protein content was >5 g/dl. The variables of acid and protein were then examined in a rabbit intestinal loop model. The hemorrhagic response in individual loops was measured using Cr51 tagged red blood cells such that the microliters of blood per centimeter intestine could be determined. Loops with organic acid and protein had significantly (p < 0.01) more intramural blood than control loops. Organic acid (possibly generated by bacterial mixed acid fermentation of carbohydrate) in the presence of protein promotes intramural hemorrhage similar to that seen in neonates with necrotizing enterocolitis.",

author = "Clark, {David A.} and Thompson, {Jeffrey E.} and Weiner, {Leonard B.} and McMillan, {Julia A.} and Schneider, {Albert J.} and Rokahr, {John E.}",

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T1 - Necrotizing enterocolitis

T2 - Intraluminal biochemistry in human neonates and a rabbit model

AU - Clark, David A.

AU - Thompson, Jeffrey E.

AU - Weiner, Leonard B.

AU - McMillan, Julia A.

AU - Schneider, Albert J.

AU - Rokahr, John E.

PY - 1985/9

Y1 - 1985/9

N2 - The intestinal contents of 17 neonates with necrotizing enterocolitis were analyzed for pH, carbohydrate, protein, and bacteria. The intraluminal pH was <5.0 (16/17). Sufficient carbohydrate and bacteria capable of fermenting the carbohydrate to organic acids were found. The intraluminal protein content was >5 g/dl. The variables of acid and protein were then examined in a rabbit intestinal loop model. The hemorrhagic response in individual loops was measured using Cr51 tagged red blood cells such that the microliters of blood per centimeter intestine could be determined. Loops with organic acid and protein had significantly (p < 0.01) more intramural blood than control loops. Organic acid (possibly generated by bacterial mixed acid fermentation of carbohydrate) in the presence of protein promotes intramural hemorrhage similar to that seen in neonates with necrotizing enterocolitis.

AB - The intestinal contents of 17 neonates with necrotizing enterocolitis were analyzed for pH, carbohydrate, protein, and bacteria. The intraluminal pH was <5.0 (16/17). Sufficient carbohydrate and bacteria capable of fermenting the carbohydrate to organic acids were found. The intraluminal protein content was >5 g/dl. The variables of acid and protein were then examined in a rabbit intestinal loop model. The hemorrhagic response in individual loops was measured using Cr51 tagged red blood cells such that the microliters of blood per centimeter intestine could be determined. Loops with organic acid and protein had significantly (p < 0.01) more intramural blood than control loops. Organic acid (possibly generated by bacterial mixed acid fermentation of carbohydrate) in the presence of protein promotes intramural hemorrhage similar to that seen in neonates with necrotizing enterocolitis.

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Necrotizing enterocolitis: Intraluminal biochemistry in human neonates and a rabbit model

Abstract

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