Naturally processed tissue- and differentiation stage-specific autologous peptides bound by HLA class I and II molecules of chronic myeloid leukemia blasts

Kyriakos P. Papadopoulos, Nicole Suciu-Foca, Charles S. Hesdorffer, Sorina Tugulea, Antonella Maffei, Paul E. Harris

Research output: Contribution to journalArticle

Abstract

Structural analysis of naturally processed peptides bound to the HLA class I and class II molecules of chronic myeloid leukemia (CML) blast cells was performed to characterize the antigen processing and autoantigen repertoire in this hematopoietic malignancy. Self-peptides derived from the carboxy-terminal end of the breakpoint cluster region (bcr) protein, as well as several differentiation stage- and tissue-specific self-antigens characteristic of early stages of myeloid differentiation, such as c-fas, c- pirn, granulocyte-macrophage colony-stimulating factor receptor a chain, proteinase 3, and cathepsin G, were identified. A common characteristic of several of the high copy-number self-peptides identified in this study is the participation of their parent proteins in signal transduction or myeloid effector function. Because bcr-abl junctional peptides bind to a limited number of major histocompatibility complex (MHC) class I alleles, an effective peptide-based immunotherapy strategy for CML requires identification of further tumor-associated or specific peptide antigens binding to common MHC alleles such as HLA-A2. The differentiation stage- and tissue-specific MHC-bound peptides found in this study, as well as the naturally processed proteins from which they are derived, may represent autoantigens towards which T-cell responses may potentially be developed for immunotherapy of hematopoietic malignancies such as CML.

Original languageEnglish (US)
Pages (from-to)4938-4946
Number of pages9
JournalBlood
Volume90
Issue number12
StatePublished - Dec 15 1997

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Papadopoulos, K. P., Suciu-Foca, N., Hesdorffer, C. S., Tugulea, S., Maffei, A., & Harris, P. E. (1997). Naturally processed tissue- and differentiation stage-specific autologous peptides bound by HLA class I and II molecules of chronic myeloid leukemia blasts. Blood, 90(12), 4938-4946.