Naturally occurring mutations define a novel function of the hepatitis B virus core promoter in core protein expression

Thomas F. Baumert, Aldo Marrone, John Vergalla, T. Jake Liang

Research output: Contribution to journalArticle

Abstract

Functional analysis of naturally occurring hepatitis B virus (HBV) mutations is crucial in understanding their impact on disease. We have recently identified two mutations in the HBV core promoter of an HBV strain associated with fulminant hepatitis leading to highly (15-fold) enhanced replication as a result of increased vital encapsidation of pregenomic RNA into the core particles (T. F. Baumert et al., J. Clin. Invest. 98:22682276, 1996). Functional studies in an encapsidation assay had demonstrated that the increase in encapsidation was largely independent of pregenomic RNA transcription. In this study, we define the molecular mechanism whereby the two core promoter mutations (C to T at nucleotide [nt] 1768 and T to A at nt 1770) result in enhanced viral encapsidation and replication. The effect of these mutations leading to increased encapsidation is mediated through enhanced core protein synthesis (15-fold) by the mutant virus. The marked increase in core protein synthesis is largely a result of posttranscriptional or translational effect of the mutations because the mutations resulted in only a twofold increase in pregenomic RNA transcription. In addition, this effect appears to be selective for core expression since reverse transcriptase-polymerase expression was increased only twofold. trans- complementation analyses of HBV replication demonstrated that enhanced replication occurred only when the mutations were provided together with the core protein in trans, confirming the functional association of the core promoter mutations and core protein expression. In addition, the effect of the mutations appears to be quantitatively dependent on the strain background to which the mutations were introduced. Our study suggests that the HBV Core promoter regulates core protein expression at both transcriptional and posttranscriptional levels.

Original languageEnglish (US)
Pages (from-to)6785-6795
Number of pages11
JournalJournal of Virology
Volume72
Issue number8
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Hepatitis B virus
protein synthesis
promoter regions
mutation
Mutation
Proteins
RNA
Nucleotides
transcription (genetics)
nucleotides
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
hepatitis
Virus Replication
virus replication
Hepatitis
Viruses
viruses
mutants

ASJC Scopus subject areas

  • Immunology

Cite this

Naturally occurring mutations define a novel function of the hepatitis B virus core promoter in core protein expression. / Baumert, Thomas F.; Marrone, Aldo; Vergalla, John; Liang, T. Jake.

In: Journal of Virology, Vol. 72, No. 8, 08.1998, p. 6785-6795.

Research output: Contribution to journalArticle

Baumert, Thomas F. ; Marrone, Aldo ; Vergalla, John ; Liang, T. Jake. / Naturally occurring mutations define a novel function of the hepatitis B virus core promoter in core protein expression. In: Journal of Virology. 1998 ; Vol. 72, No. 8. pp. 6785-6795.
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