Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae

Chris M. Cirimotich, Yuemei Dong, April M. Clayton, Simone L. Sandiford, Jayme A. Souza-Neto, Musapa Mulenga, George Dimopoulos

Research output: Contribution to journalArticle

Abstract

Malaria parasite transmission depends on the successful transition of Plasmodium through discrete developmental stages in the lumen of the mosquito midgut. Like the human intestinal tract, the mosquito midgut contains a diverse microbial flora, which may compromise the ability of Plasmodium to establish infection. We have identified an Enterobacter bacterium isolated from wild mosquito populations in Zambia that renders the mosquito resistant to infection with the human malaria parasite Plasmodium falciparum by interfering with parasite development before invasion of themidgut epithelium. Phenotypic analyses showed that the anti-Plasmodium mechanism requires small populations of replicating bacteria and is mediated through a mosquito-independent interaction with the malaria parasite. We show that this anti-Plasmodium effect is largely caused by bacterial generation of reactive oxygen species.

Original languageEnglish (US)
Pages (from-to)855-858
Number of pages4
JournalScience
Volume332
Issue number6031
DOIs
StatePublished - May 13 2011

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Anopheles gambiae
Culicidae
Plasmodium
Malaria
Parasites
Bacteria
Zambia
Enterobacter
Falciparum Malaria
Infection
Population
Reactive Oxygen Species
Epithelium

ASJC Scopus subject areas

  • General

Cite this

Cirimotich, C. M., Dong, Y., Clayton, A. M., Sandiford, S. L., Souza-Neto, J. A., Mulenga, M., & Dimopoulos, G. (2011). Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae. Science, 332(6031), 855-858. https://doi.org/10.1126/science.1201618

Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae. / Cirimotich, Chris M.; Dong, Yuemei; Clayton, April M.; Sandiford, Simone L.; Souza-Neto, Jayme A.; Mulenga, Musapa; Dimopoulos, George.

In: Science, Vol. 332, No. 6031, 13.05.2011, p. 855-858.

Research output: Contribution to journalArticle

Cirimotich, CM, Dong, Y, Clayton, AM, Sandiford, SL, Souza-Neto, JA, Mulenga, M & Dimopoulos, G 2011, 'Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae', Science, vol. 332, no. 6031, pp. 855-858. https://doi.org/10.1126/science.1201618
Cirimotich CM, Dong Y, Clayton AM, Sandiford SL, Souza-Neto JA, Mulenga M et al. Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae. Science. 2011 May 13;332(6031):855-858. https://doi.org/10.1126/science.1201618
Cirimotich, Chris M. ; Dong, Yuemei ; Clayton, April M. ; Sandiford, Simone L. ; Souza-Neto, Jayme A. ; Mulenga, Musapa ; Dimopoulos, George. / Natural microbe-mediated refractoriness to Plasmodium infection in Anopheles gambiae. In: Science. 2011 ; Vol. 332, No. 6031. pp. 855-858.
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