The interactions of Na+ and Ca++ were examined in synaptosomes and mitochondria. Na+ decreased both 45Ca++ binding to synaptosomes and loss of 45Ca++ from mitochondria. The ability of low concentrations of a local anesthetic, N,N-diethylamino-octyl 3,4,5-trimethoxybenzoate, to inhibit movement of Ca from intracellular bound stores was found to be associated with increased uptake of Ca by synaptosomes. A Na+-dependent regulatory effect was postulated between mitochondrial binding of Ca and influx of Ca into the nerve terminal. In addition, the results suggest difficulties in distinguishing between Na+-dependent and Ca++-dependent processes in synaptosomal function.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 25 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology