Narp and NP1 form heterocomplexes that function in developmental and activity-dependent synaptic plasticity

Desheng Xu, Carsten Hopf, Radhika Reddy, Richard W. Cho, Liping Guo, Anthony Lanahan, Ronald S. Petralia, Robert J. Wenthold, Richard J. O'Brien, Paul F Worley

Research output: Contribution to journalArticle

Abstract

Narp is a neuronal immediate early gene that plays a role in excitatory synaptogenesis. Here, we report that native Narp in brain is part of a pentraxin complex that includes NP1. These proteins are covalently linked by disulfide bonds into highly organized complexes, and their relative ratio in the complex is dynamically dependent upon the neuron's activity history and developmental stage. Complex formation is dependent on their distinct N-terminal coiled-coil domains, while their closely homologous C-terminal pentraxin domains mediate association with AMPA-type glutamate receptors. Narp is substantially more effective in assays of cell surface cluster formation, coclustering of AMPA receptors, and excitatory synaptogenesis, yet their combined expression results in supraadditive effects. These studies support a model in which Narp can regulate the latent synaptogenic activity of NP1 by forming mixed pentraxin assemblies. This mechanism appears to contribute to both activity-independent and activity-dependent excitatory synaptogenesis.

Original languageEnglish (US)
Pages (from-to)513-528
Number of pages16
JournalNeuron
Volume39
Issue number3
DOIs
StatePublished - Jul 31 2003

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Neuronal Plasticity
AMPA Receptors
Immediate-Early Genes
Glutamate Receptors
Disulfides
History
Neurons
Brain
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Narp and NP1 form heterocomplexes that function in developmental and activity-dependent synaptic plasticity. / Xu, Desheng; Hopf, Carsten; Reddy, Radhika; Cho, Richard W.; Guo, Liping; Lanahan, Anthony; Petralia, Ronald S.; Wenthold, Robert J.; O'Brien, Richard J.; Worley, Paul F.

In: Neuron, Vol. 39, No. 3, 31.07.2003, p. 513-528.

Research output: Contribution to journalArticle

Xu, D, Hopf, C, Reddy, R, Cho, RW, Guo, L, Lanahan, A, Petralia, RS, Wenthold, RJ, O'Brien, RJ & Worley, PF 2003, 'Narp and NP1 form heterocomplexes that function in developmental and activity-dependent synaptic plasticity', Neuron, vol. 39, no. 3, pp. 513-528. https://doi.org/10.1016/S0896-6273(03)00463-X
Xu, Desheng ; Hopf, Carsten ; Reddy, Radhika ; Cho, Richard W. ; Guo, Liping ; Lanahan, Anthony ; Petralia, Ronald S. ; Wenthold, Robert J. ; O'Brien, Richard J. ; Worley, Paul F. / Narp and NP1 form heterocomplexes that function in developmental and activity-dependent synaptic plasticity. In: Neuron. 2003 ; Vol. 39, No. 3. pp. 513-528.
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AU - Lanahan, Anthony

AU - Petralia, Ronald S.

AU - Wenthold, Robert J.

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