Nanostructure of supported phospholipid monolayers and bilayers by scanning probe microscopy

Lukas K. Tamm, Christine Böhm, Jie Yang, Zhifeng Shao, Jeeseong Hwang, Michael Edidin, Eric Betzig

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Near-field scanning optical and atomic force microscopy were used to image structural details of supported lipid monolayers and bilayers. Compressed DPPC monolayers exhibited dense web-like structures whereas DPPE monolayers were more uniform with elongated inclusion domains of fluorescent probes. When a second lipid monolayer was self-assembled on a supported monolayer [Kalb et al., Biochim. Biophys. Acta 1103 (1992) 307], the structure of the resulting supported bilayer strongly depended on the coupling monolayer. On coupling monolayers of DPPC, the bilayers exhibited domains with finger-like extensions. Much more uniform bilayers were obtained when the coupling layer was DPPE. Towards the goal of developing a biosensor for the detection of viruses in biological fluids, bilayers were constructed with 10 mol% of the ganglioside GD1a in the outer monolayer. Specific binding of influenza viruses to GD1a receptors was monitored by total internal reflection fluorescence and atomic force microscopy.

Original languageEnglish (US)
Pages (from-to)813-816
Number of pages4
JournalThin Solid Films
StatePublished - Sep 15 1996


  • AFM
  • Biosensor
  • NSOM
  • Supported bilayer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


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