Nanoscale artificial antigen presenting cells for T cell immunotherapy

Karlo Perica, Andrés De León Medero, Malarvizhi Durai, Yen Ling Chiu, Joan Glick Bieler, Leah Sibener, Michaela Niemöller, Mario Assenmacher, Anne Richter, Michael Edidin, Mathias Oelke, Jonathan Schneck

Research output: Contribution to journalArticle

Abstract

Artificial antigen presenting cells (aAPC), which deliver stimulatory signals to cytotoxic lymphocytes, are a powerful tool for both adoptive and active immunotherapy. Thus far, aAPC have been synthesized by coupling T cell activating proteins such as CD3 or MHC-peptide to micron-sized beads. Nanoscale platforms have different trafficking and biophysical interaction properties and may allow development of new immunotherapeutic strategies. We therefore manufactured aAPC based on two types of nanoscale particle platforms: biocompatible iron-dextran paramagnetic particles (50-100. nm in diameter) and avidin-coated quantum dot nanocrystals (~. 30. nm). Nanoscale aAPC induced antigen-specific T cell proliferation from mouse splenocytes and human peripheral blood T cells. When injected in vivo, both iron-dextran particles and quantum dot nanocrystals enhanced tumor rejection in a subcutaneous mouse melanoma model. This is the first description of nanoscale aAPC that induce antigen-specific T cell proliferation in vitro and lead to effective T cell stimulation and inhibition of tumor growth in vivo. From the Clinical Editor: Artifical antigen presenting cells could revolutionize the field of cancer-directed immunotherapy. This team of investigators have manufactured two types of nanoscale particle platform-based aAPCs and demonstrates that both iron-dextran particles and quantum dot nanocrystals enhance tumor rejection in a melanoma model, providing the first description of nanoscale aAPCs that lead to effective T cell stimulation and inhibition of tumor growth.

Original languageEnglish (US)
Pages (from-to)119-129
Number of pages11
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2014

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Keywords

  • Artificial antigen presenting cell
  • Immunotherapy
  • Nanoparticle
  • T cell

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Perica, K., De León Medero, A., Durai, M., Chiu, Y. L., Bieler, J. G., Sibener, L., Niemöller, M., Assenmacher, M., Richter, A., Edidin, M., Oelke, M., & Schneck, J. (2014). Nanoscale artificial antigen presenting cells for T cell immunotherapy. Nanomedicine: Nanotechnology, Biology, and Medicine, 10(1), 119-129. https://doi.org/10.1016/j.nano.2013.06.015