Nanophthalmic Sclera: Fibronectin Studies

Beatrice Y.J.T. Yue, Akimitsu Kurosawa, Josephine Duvall, Morton F. Goldberg, Mark O.M. Tso, Joel Sugar

Research output: Contribution to journalArticlepeer-review

Abstract

The authors performed fibronectin studies on scleral specimens derived from a patient with nanophthalmos. Immunohistochemical staining with antifibronectin was conducted using both formaldehyde-fixed, paraffin-embedded tissue sections and unfixed tissue-cultured scleral cells. In each case, the nanophthalmic samples exhibited fibronectin staining stronger than that obtained from normal human subjects. Results from an enzyme-linked immunosorbent assay (ELISA) confirmed the histologic findings that, in tissue culture, the patient's scleral cells contained and secreted a higher amount of fibronectin than did the normal control cells. The elevated fibronectin level may be related to the development of nanophthalmos.

Original languageEnglish (US)
Pages (from-to)56-60
Number of pages5
JournalOphthalmology
Volume95
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Keywords

  • cultures
  • enzyme-linked immunosorbent assay
  • fibronectin
  • immunohistochemical staining
  • nanophthalmos
  • sclera

ASJC Scopus subject areas

  • Ophthalmology

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