Nanoparticles for gene transfer to human embryonic stem cell colonies

Jordan J. Green, Betty Y. Zhou, Maisam M. Mitalipova, Caroline Beard, Robert Langer, Rudolf Jaenisch, Daniel G. Anderson

Research output: Contribution to journalArticlepeer-review

Abstract

We develop biodegradable polymeric nanoparticles to facilitate nonvirai gene transfer to human embryonic stem cells (hESCs). Small (∼200 nm), positively charged (∼10 mV) particles are formed by the self assembly of cationic, hydrolytically degradable poly(β-amino esters) and plasmid DNA. By varying the end group of the polymer, we can tune the biophysical properties of the resulting nanoparticles and their gene-delivery efficacy. We created an OCT4-driven GFP hES cell line to allow the rapid identification of nanoparticles that facilitate gene transfer while maintaining an hESC undifferentiated state. Using this cell system, we synthesized nanoparticles that have gene delivery efficacy that is up to 4 times higher than that of the leading commercially available transfection agent, Lipofectamine 2000. Importantly, these materials have minimal toxicity and do not adversely affect hESC colony morphology or cause nonspecific differentiation.

Original languageEnglish (US)
Pages (from-to)3126-3130
Number of pages5
JournalNano Letters
Volume8
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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