Nanoparticles for gene transfer to human embryonic stem cell colonies

Jordan J. Green; Betty Y. Zhou; Maisam M. Mitalipova; Caroline Beard; Robert Langer; Rudolf Jaenisch; Daniel G. Anderson

doi:10.1021/nl8012665

Nanoparticles for gene transfer to human embryonic stem cell colonies

Jordan J. Green, Betty Y. Zhou, Maisam M. Mitalipova, Caroline Beard, Robert Langer, Rudolf Jaenisch, Daniel G. Anderson

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

We develop biodegradable polymeric nanoparticles to facilitate nonvirai gene transfer to human embryonic stem cells (hESCs). Small (∼200 nm), positively charged (∼10 mV) particles are formed by the self assembly of cationic, hydrolytically degradable poly(β-amino esters) and plasmid DNA. By varying the end group of the polymer, we can tune the biophysical properties of the resulting nanoparticles and their gene-delivery efficacy. We created an OCT4-driven GFP hES cell line to allow the rapid identification of nanoparticles that facilitate gene transfer while maintaining an hESC undifferentiated state. Using this cell system, we synthesized nanoparticles that have gene delivery efficacy that is up to 4 times higher than that of the leading commercially available transfection agent, Lipofectamine 2000. Importantly, these materials have minimal toxicity and do not adversely affect hESC colony morphology or cause nonspecific differentiation.

Original language	English (US)
Pages (from-to)	3126-3130
Number of pages	5
Journal	Nano Letters
Volume	8
Issue number	10
DOIs	https://doi.org/10.1021/nl8012665
State	Published - Oct 2008
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl8012665

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title = "Nanoparticles for gene transfer to human embryonic stem cell colonies",

abstract = "We develop biodegradable polymeric nanoparticles to facilitate nonvirai gene transfer to human embryonic stem cells (hESCs). Small (∼200 nm), positively charged (∼10 mV) particles are formed by the self assembly of cationic, hydrolytically degradable poly(β-amino esters) and plasmid DNA. By varying the end group of the polymer, we can tune the biophysical properties of the resulting nanoparticles and their gene-delivery efficacy. We created an OCT4-driven GFP hES cell line to allow the rapid identification of nanoparticles that facilitate gene transfer while maintaining an hESC undifferentiated state. Using this cell system, we synthesized nanoparticles that have gene delivery efficacy that is up to 4 times higher than that of the leading commercially available transfection agent, Lipofectamine 2000. Importantly, these materials have minimal toxicity and do not adversely affect hESC colony morphology or cause nonspecific differentiation.",

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T1 - Nanoparticles for gene transfer to human embryonic stem cell colonies

AU - Green, Jordan J.

AU - Zhou, Betty Y.

AU - Mitalipova, Maisam M.

AU - Beard, Caroline

AU - Langer, Robert

AU - Jaenisch, Rudolf

AU - Anderson, Daniel G.

PY - 2008/10

Y1 - 2008/10

N2 - We develop biodegradable polymeric nanoparticles to facilitate nonvirai gene transfer to human embryonic stem cells (hESCs). Small (∼200 nm), positively charged (∼10 mV) particles are formed by the self assembly of cationic, hydrolytically degradable poly(β-amino esters) and plasmid DNA. By varying the end group of the polymer, we can tune the biophysical properties of the resulting nanoparticles and their gene-delivery efficacy. We created an OCT4-driven GFP hES cell line to allow the rapid identification of nanoparticles that facilitate gene transfer while maintaining an hESC undifferentiated state. Using this cell system, we synthesized nanoparticles that have gene delivery efficacy that is up to 4 times higher than that of the leading commercially available transfection agent, Lipofectamine 2000. Importantly, these materials have minimal toxicity and do not adversely affect hESC colony morphology or cause nonspecific differentiation.

AB - We develop biodegradable polymeric nanoparticles to facilitate nonvirai gene transfer to human embryonic stem cells (hESCs). Small (∼200 nm), positively charged (∼10 mV) particles are formed by the self assembly of cationic, hydrolytically degradable poly(β-amino esters) and plasmid DNA. By varying the end group of the polymer, we can tune the biophysical properties of the resulting nanoparticles and their gene-delivery efficacy. We created an OCT4-driven GFP hES cell line to allow the rapid identification of nanoparticles that facilitate gene transfer while maintaining an hESC undifferentiated state. Using this cell system, we synthesized nanoparticles that have gene delivery efficacy that is up to 4 times higher than that of the leading commercially available transfection agent, Lipofectamine 2000. Importantly, these materials have minimal toxicity and do not adversely affect hESC colony morphology or cause nonspecific differentiation.

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Nanoparticles for gene transfer to human embryonic stem cell colonies

Abstract

ASJC Scopus subject areas

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