Nanoparticles coated with high molecular weight PEG penetrate mucus and provide uniform vaginal and colorectal distribution in vivo

Katharina Maisel, Mihika Reddy, Qingguo Xu, Sumon Chattopadhyay, Richard Cone, Laura Ensign-Hodges, Justin S Hanes

Research output: Contribution to journalArticle

Abstract

Aim: We previously reported that nanoparticles (NPs) coated with 10 kDa PEG were mucoadhesive. Here, we demonstrate that by increasing the surface density, PEG with molecular weight (MW) as high as 40 kDa can be used as a mucoinert NP surface coating. Materials & methods: We compared two sets of reaction conditions for coating model polystyrene NPs with 10 kDa PEG and used optimized conditions to coat NPs with PEG as high as 40 kDa in MW. We then characterized NP transport in human cervicovaginal mucus ex vivo. We further administered PEG-coated NPs to the mouse cervicovaginal tract and colorectum to assess mucosal distribution in vivo. Results & conclusion: We demonstrate here that PEG with MW as high as 40 kDa can be densely grafted to the surface of NP to prevent interactions with mucus. NP coated with 10-40 kDa PEG rapidly diffused through human cervicovaginal mucus ex vivo, and uniformly lined the mouse colorectal and vaginal epithelium in vivo.

Original languageEnglish (US)
Pages (from-to)1337-1343
Number of pages7
JournalNanomedicine
Volume11
Issue number11
DOIs
StatePublished - Jun 1 2016

Keywords

  • hypotonic delivery
  • mucosal drug delivery
  • mucus penetrating nanoparticles (MPPs)
  • PEG density
  • PEG molecular weight

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

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