Nanoparticle tracking analysis for determination of hydrodynamic diameter, concentration, and zeta-potential of polyplex nanoparticles

David R. Wilson, Jordan J. Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nanoparticle tracking analysis (NTA) is a recently developed nanoparticle characterization technique that offers certain advantages over dynamic light scattering for characterizing polyplex nanoparticles in particular. Dynamic light scattering results in intensity-weighted average measurements of nanoparticle characteristics. In contrast, NTA directly tracks individual particles, enabling concentration measurements as well as the direct determination of number-weighted particle size and zeta-potential. A direct number-weighted assessment of nanoparticle characteristics is particularly useful for polydisperse samples of particles, including many varieties of gene delivery particles that can be prone to aggregation. Here, we describe the synthesis of poly(beta-amino ester)/deoxyribonucleic acid (PBAE/DNA) polyplex nanoparticles and their characterization using NTA to determine hydrodynamic diameter, zeta-potential, and concentration. Additionally, we detail methods of labeling nucleic acids with fluorophores to assess only those polyplex nanoparticles containing plasmids via NTA. Polymeric gene delivery of exogenous plasmid DNA has great potential for treating a wide variety of diseases by inducing cells to express a gene of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-46
Number of pages16
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1570
ISSN (Print)1064-3745

Keywords

  • Concentration
  • DNA labeling
  • Hydrodynamic diameter
  • Nanoparticle tracking analysis
  • Zeta-potential

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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