Nanofibrous nerve conduit-enhanced peripheral nerve regeneration

Xu Jiang, Ruifa Mi, Ahmet Hoke, Sing Yian Chew

Research output: Contribution to journalArticle

Abstract

Fibre structures represent a potential class of materials for the formation of synthetic nerve conduits due to their biomimicking architecture. Although the advantages of fibres in enhancing nerve regeneration have been demonstrated, in vivo evaluation of fibre size effect on nerve regeneration remains limited. In this study, we analyzed the effects of fibre diameter of electrospun conduits on peripheral nerve regeneration across a 15-mm critical defect gap in a rat sciatic nerve injury model. By using an electrospinning technique, fibrous conduits comprised of aligned electrospun poly (ε-caprolactone) (PCL) microfibers (981±83 nm, Microfiber) or nanofibers (251±32 nm, Nanofiber) were obtained. At three months post implantation, axons regenerated across the defect gap in all animals that received fibrous conduits. In contrast, complete nerve regeneration was not observed in the control group that received empty, non-porous PCL film conduits (Film). Nanofiber conduits resulted in significantly higher total number of myelinated axons and thicker myelin sheaths compared to Microfiber and Film conduits. Retrograde labeling revealed a significant increase in number of regenerated dorsal root ganglion sensory neurons in the presence of Nanofiber conduits (1.93 ± 0.71 × 103 vs. 0.98 ± 0.30 × 103 in Microfiber, p

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume8
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Nanofibers
Nerve Regeneration
Peripheral Nerves
Fibers
Axons
Defects
Electrospinning
Spinal Ganglia
Sensory Receptor Cells
Sciatic Nerve
Myelin Sheath
Labeling
Neurons
Rats
Animals
Control Groups
Wounds and Injuries

Keywords

  • Contact guidance
  • Critical defect gap
  • Electrospinning
  • Nanofibers
  • Neural tissue engineering
  • Sciatic nerve regeneration

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)

Cite this

Nanofibrous nerve conduit-enhanced peripheral nerve regeneration. / Jiang, Xu; Mi, Ruifa; Hoke, Ahmet; Chew, Sing Yian.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 8, No. 5, 2014, p. 377-385.

Research output: Contribution to journalArticle

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