Nanoelectromechanics of methylated DNA in a synthetic nanopore

U. Mirsaidov; W. Timp; X. Zou; V. Dimitrov; K. Schulten; A. P. Feinberg; G. Timp

doi:10.1016/j.bpj.2008.12.3760

Nanoelectromechanics of methylated DNA in a synthetic nanopore

U. Mirsaidov, W. Timp, X. Zou, V. Dimitrov, K. Schulten, A. P. Feinberg, G. Timp

Research output: Contribution to journal › Article › peer-review

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Abstract

Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

Original language	English (US)
Pages (from-to)	L32-L34
Journal	Biophysical journal
Volume	96
Issue number	4
DOIs	https://doi.org/10.1016/j.bpj.2008.12.3760
State	Published - Feb 18 2009

ASJC Scopus subject areas

Biophysics

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10.1016/j.bpj.2008.12.3760

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title = "Nanoelectromechanics of methylated DNA in a synthetic nanopore",

abstract = "Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.",

author = "U. Mirsaidov and W. Timp and X. Zou and V. Dimitrov and K. Schulten and Feinberg, {A. P.} and G. Timp",

note = "Funding Information: We acknowledge the use of the Center for Microanalysis of Materials, supported by the United States Department of Energy grant No. DEFG02-91-ER45439. This work was funded by National Institutes of Health grant Nos. R01 HG003713A and P41-RR05969, and National Science Foundation grant No. TH 2008-01040 ANTC; it was also partially supported by National Institutes of Health grant No. CA65145. ",

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AU - Mirsaidov, U.

AU - Timp, W.

AU - Zou, X.

AU - Dimitrov, V.

AU - Schulten, K.

AU - Feinberg, A. P.

AU - Timp, G.

N1 - Funding Information: We acknowledge the use of the Center for Microanalysis of Materials, supported by the United States Department of Energy grant No. DEFG02-91-ER45439. This work was funded by National Institutes of Health grant Nos. R01 HG003713A and P41-RR05969, and National Science Foundation grant No. TH 2008-01040 ANTC; it was also partially supported by National Institutes of Health grant No. CA65145.

PY - 2009/2/18

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N2 - Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

AB - Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

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Nanoelectromechanics of methylated DNA in a synthetic nanopore

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