Nano-patterned SU-8 surface using nanosphere-lithography for enhanced neuronal cell growth

Eunhee Kim, Seung Jun Yoo, Eunjung Kim, Tae Hwan Kwon, Li Zhang, Cheil Moon, Hongsoo Choi

Research output: Contribution to journalArticlepeer-review

Abstract

Mimicking the nanoscale surface texture of the extracellular matrix can affect the regulation of cellular behavior, including adhesion, differentiation, and neurite outgrowth. In this study, SU-8-based polymer surfaces with well-ordered nanowell arrays were fabricated using nanosphere lithography with polystyrene nanoparticles. We show that the SU-8 surface with nanowells resulted in similar neuronal development of rat pheochromocytoma (PC12) cells compared with an unpatterned poly-L-lysine (PLL)-coated SU-8 surface. Additionally, even after soaking the substrate in cell culture medium for two weeks, cells on the nanowell SU-8 surface showed long-term neurite outgrowth compared to cells on the PLL-coated SU-8 surface. The topographical surface modification of the nanowell array demonstrates potential as a replacement for cell adhesive material coatings such as PLL, for applications requiring long-term use of polymer-based implantable devices.

Original languageEnglish (US)
Article number175303
JournalNanotechnology
Volume27
Issue number17
DOIs
StatePublished - Mar 17 2016
Externally publishedYes

Keywords

  • nano-patterned
  • nanosphere lithography
  • neurite outgrowth
  • surface modification

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

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