NAD+-mediated stimulation of adenylate cyclase in cardiac membranes

Arthur M. Feldman, Michael A. Levine, Kenneth L. Baughman, Cornelis Van Dop

    Research output: Contribution to journalArticle

    Abstract

    NAD+ significantly enhances adenylate cyclase activity in crude cardiac membrane preparations. This increase is dose-dependent, does not occur in the presence of nicotinamide, ADP-ribose or NADP+, and can be effected by a 30 min pre-incubation period with NAD+. Time course studies are consistent with an enzymatically mediated modification that uses NAD+ as substrate. Furthermore, inhibition of the NAD+-mediated activation by arginine suggests that this modulation of cardiac adenylate cyclase is analogous to that catalyzed by endogenous ADP-ribosyl transferases.

    Original languageEnglish (US)
    Pages (from-to)631-637
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume142
    Issue number3
    DOIs
    StatePublished - Feb 13 1987

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

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