NaCl uptake by the branchial epithelium in freshwater teleost fish: An immunological approach to ion-transport protein localization

Jonathan M. Wilson, Pierre Laurent, Bruce L. Tufts, Dale J. Benos, Mark Donowitz, A. Wayne Vogl, David J. Randall

Research output: Contribution to journalArticlepeer-review

Abstract

Teleost fishes, living in fresh water, engage in active ion uptake to maintain ion homeostasis. Current models for NaCl uptake involve Na+ uptake via an apical amiloride-sensitive epithelial Na+ channel (ENaC), energized by an apical vacuolar-type proton pump (V-ATPase) or alternatively by an amiloride-sensitive Na+/H+ exchange (NHE) protein, and apical Cl- uptake mediated by an electroneutral, SITS-sensitive Cl-/HCO3- anion-exchange protein. Using non-homologous antibodies, we have determined the cellular distributions of these ion-transport proteins to test the predicted models. Na+/K+-ATPase was used as a cellular marker for differentiating branchial epithelium mitochondria-rich (MR) cells from pavement cells. In both the freshwater tilapia (Oreochromis mossambicus) and rainbow trout (Oncorhynchus mykiss), V-ATPase and ENaC-like immunoreactivity co-localized to pavement cells, although apical labelling was also found in MR cells in the trout. In the freshwater tilapia, apical anion-exchanger-like immunoreactivity is found in the MR cells. Thus, a freshwater-type MR chloride cell exists in teleost fishes. The NHE-like immunoreactivity is associated with the accessory cell type and with a small population of pavement cells in tilapia.

Original languageEnglish (US)
Pages (from-to)2279-2296
Number of pages18
JournalJournal of Experimental Biology
Volume203
Issue number15
StatePublished - Aug 1 2000

Keywords

  • Anion exchanger
  • Epithelial Na channel
  • Fish
  • Gill
  • H-ATPase, Na/K-ATPase
  • Ion transport
  • Mitochondria-rich cell
  • Na/H exchanger
  • Oncorhynchus mykiss
  • Oreochromis mossambicus
  • Pavement cell
  • Rainbow trout
  • Tilapia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Fingerprint Dive into the research topics of 'NaCl uptake by the branchial epithelium in freshwater teleost fish: An immunological approach to ion-transport protein localization'. Together they form a unique fingerprint.

Cite this