NAADP Activates two-pore channels on t cell cytolytic granules to stimulate exocytosis and killing

Lianne C. Davis, Anthony J. Morgan, Ji Li Chen, Charlotte M. Snead, Duncan Bloor-Young, Eugene Shenderov, Megan N. Stanton-Humphreys, Stuart J. Conway, Grant C. Churchill, John Parrington, Vincenzo Cerundolo, Antony Galione

Research output: Contribution to journalArticle

Abstract

A cytotoxic T lymphocyte (CTL) kills an infected or tumorigenic cell by Ca2+-dependent exocytosis of cytolytic granules at the immunological synapse formed between the two cells. Although inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release from the endoplasmic reticulum activates the store-operated Ca2+-influx pathway that is necessary for exocytosis, it is not a sufficient stimulus [1-4]. Here we identify the Ca2+-mobilizing messenger nicotinic acid adenine dinucleotide phosphate (NAADP) and its recently identified molecular target, two-pore channels (TPCs) [5-7], as being important for T cell receptor signaling in CTLs. We demonstrate that cytolytic granules are not only reservoirs of cytolytic proteins but are also the acidic Ca2+ stores mobilized by NAADP via TPC channels on the granules themselves, so that TPCs migrate to the immunological synapse upon CTL activation. Moreover, NAADP activates TPCs to drive exocytosis in a way that is not mimicked by global Ca2+ signals induced by IP3 or ionomycin, suggesting that critical, local Ca 2+ nanodomains around TPCs stimulate granule exocytosis. Hence, by virtue of the NAADP/TPC pathway, cytolytic granules generate Ca2+ signals that lead to their own exocytosis and to cell killing. This study highlights a selective role for NAADP in stimulating exocytosis crucial for immune cell function and may impact on stimulus-secretion coupling in wider cellular contexts.

Original languageEnglish (US)
Pages (from-to)2331-2337
Number of pages7
JournalCurrent Biology
Volume22
Issue number24
DOIs
StatePublished - Dec 18 2012
Externally publishedYes

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exocytosis
Exocytosis
niacin
adenine
granules
phosphates
calcium
Immunological Synapses
T-cells
Cytotoxic T-Lymphocytes
cells
cytotoxic T-lymphocytes
Ionomycin
Inositol 1,4,5-Trisphosphate
T-Cell Antigen Receptor
Lymphocyte Activation
Endoplasmic Reticulum
Chemical activation
lymphocyte proliferation
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Davis, L. C., Morgan, A. J., Chen, J. L., Snead, C. M., Bloor-Young, D., Shenderov, E., ... Galione, A. (2012). NAADP Activates two-pore channels on t cell cytolytic granules to stimulate exocytosis and killing. Current Biology, 22(24), 2331-2337. https://doi.org/10.1016/j.cub.2012.10.035

NAADP Activates two-pore channels on t cell cytolytic granules to stimulate exocytosis and killing. / Davis, Lianne C.; Morgan, Anthony J.; Chen, Ji Li; Snead, Charlotte M.; Bloor-Young, Duncan; Shenderov, Eugene; Stanton-Humphreys, Megan N.; Conway, Stuart J.; Churchill, Grant C.; Parrington, John; Cerundolo, Vincenzo; Galione, Antony.

In: Current Biology, Vol. 22, No. 24, 18.12.2012, p. 2331-2337.

Research output: Contribution to journalArticle

Davis, LC, Morgan, AJ, Chen, JL, Snead, CM, Bloor-Young, D, Shenderov, E, Stanton-Humphreys, MN, Conway, SJ, Churchill, GC, Parrington, J, Cerundolo, V & Galione, A 2012, 'NAADP Activates two-pore channels on t cell cytolytic granules to stimulate exocytosis and killing', Current Biology, vol. 22, no. 24, pp. 2331-2337. https://doi.org/10.1016/j.cub.2012.10.035
Davis, Lianne C. ; Morgan, Anthony J. ; Chen, Ji Li ; Snead, Charlotte M. ; Bloor-Young, Duncan ; Shenderov, Eugene ; Stanton-Humphreys, Megan N. ; Conway, Stuart J. ; Churchill, Grant C. ; Parrington, John ; Cerundolo, Vincenzo ; Galione, Antony. / NAADP Activates two-pore channels on t cell cytolytic granules to stimulate exocytosis and killing. In: Current Biology. 2012 ; Vol. 22, No. 24. pp. 2331-2337.
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