(Na + K +)-ATPase is a target for phosphoinositide 3-kinase/ protein kinase B and protein kinase C pathways triggered by albumin

Diogo B. Peruchetti, Ana Acacia S. Pinheiro, Sharon S. Landgraf, Mira Wengert, Christina M. Takiya, William B. Guggino, Celso Caruso-Neves

Research output: Contribution to journalArticle

Abstract

In recent decades, evidence has confirmed the crucial role of albumin in the progression of renal disease. However, the possible role of signaling pathways triggered by physiologic concentrations of albumin in the modulation of proximal tubule (PT) sodium reabsorption has not been considered. In the present work, we have shown that a physiologic concentration of albumin increases the expression of the α1 subunit of (Na + + K +)- ATPase in LLC-PK1 cells leading to an increase in enzyme activity. This process involves the sequential activation of PI3K/ protein kinase B and protein kinase C pathways promoting inhibition of protein kinase A. This integrative network is inhibited when albumin concentration is increased, similar to renal disease, leading to a decrease in the α1 subunit of (Na + + K +)- ATPase expression. Together, the results indicate that variation in albumin concentration in PT cells has an important effect on PT sodium reabsorption and, consequently, on renal sodium excretion.

Original languageEnglish (US)
Pages (from-to)45041-45047
Number of pages7
JournalJournal of Biological Chemistry
Volume286
Issue number52
DOIs
StatePublished - Dec 30 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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