Naïve Bayesian Models for Vero Cell Cytotoxicity

Alexander L. Perryman, Jimmy S. Patel, Riccardo Russo, Eric Singleton, Nancy Connell, Sean Ekins, Joel S. Freundlich

Research output: Contribution to journalArticle

Abstract

Purpose: To advance translational research of potential therapeutic small molecules against infectious microbes, the compounds must display a relative lack of mammalian cell cytotoxicity. Vero cell cytotoxicity (CC50) is a common initial assay for this metric. We explored the development of naïve Bayesian models that can enhance the probability of identifying non-cytotoxic compounds. Methods: Vero cell cytotoxicity assays were identified in PubChem, reformatted, and curated to create a training set with 8741 unique small molecules. These data were used to develop Bayesian classifiers, which were assessed with internal cross-validation, external tests with a set of 193 compounds from our laboratory, and independent validation with an additional diverse set of 1609 unique compounds from PubChem. Results: Evaluation with independent, external test and validation sets indicated that cytotoxicity Bayesian models constructed with the ECFP_6 descriptor were more accurate than those that used FCFP_6 fingerprints. The best cytotoxicity Bayesian model displayed predictive power in external evaluations, according to conventional and chance-corrected statistics, as well as enrichment factors. Conclusions: The results from external tests demonstrate that our novel cytotoxicity Bayesian model displays sufficient predictive power to help guide translational research. To assist the chemical tool and drug discovery communities, our curated training set is being distributed as part of the Supplementary Material. [Figure not available: see fulltext.].

Original languageEnglish (US)
Article number170
JournalPharmaceutical Research
Volume35
Issue number9
DOIs
StatePublished - Sep 1 2018
Externally publishedYes

Keywords

  • Bayesian model
  • machine learning
  • predicting mammalian cytotoxicity
  • translational research
  • vero cell CC

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

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  • Cite this

    Perryman, A. L., Patel, J. S., Russo, R., Singleton, E., Connell, N., Ekins, S., & Freundlich, J. S. (2018). Naïve Bayesian Models for Vero Cell Cytotoxicity. Pharmaceutical Research, 35(9), [170]. https://doi.org/10.1007/s11095-018-2439-9