N-methyl-d-aspartate receptor independent changes in expression of polysialic acid-neural cell adhesion molecule despite blockade of homosynaptic long-term potentiation and heterosynaptic long-term depression in the awake freely behaving rat dentate gyrus

Jose J. Rodríguez, Glenn M. Dallérac, Masashi Tabuchi, Heather A. Davies, Frances M. Colyer, Michael G. Stewart, Valérie Doyère

Research output: Contribution to journalArticle

Abstract

Investigations examining the role of polysialic acid (PSA) on the neural cell adhesion molecule (NCAM) in synaptic plasticity have yielded inconsistent data. Here, we addressed this issue by determining whether homosynaptic long-term potentiation (LTP) and heterosynaptic long-term depression (LTD) induce changes in the distribution of PSA-NCAM in the dentate gyrus (DG) of rats in vivo. In addition, we also examined whether the observed modifications were initiated via the activation of N-methyl-d-aspartate (NMDA) receptors. Immunocytochemical analysis showed an increase in PSA-NCAM positive cells both at 2 and 24h following high-frequency stimulation of either medial or lateral perforant paths, leading to homosynaptic LTP and heterosynaptic LTD, respectively, in the medial molecular layer of the DG. Analysis of sub-cellular distribution of PSA-NCAM by electron microscopy showed decreased PSA dendritic labelling in LTD rats and a sub-cellular relocation towards the spines in LTP rats. Importantly, these modifications were found to be independent of the activation of NMDA receptors. Our findings suggest that strong activation of the granule cells up-regulates PSA-NCAM synthesis which then incorporates into activated synapses, representing NMDA-independent plastic processes that act synergistically on LTP/LTD mechanisms without participating in their expression.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalNeuron Glia Biology
Volume4
Issue number3
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

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Neural Cell Adhesion Molecules
Long-Term Potentiation
Dentate Gyrus
Perforant Pathway
Neuronal Plasticity
Aspartic Acid
Synapses
aspartic acid receptor
polysialic acid
Electron Microscopy
Spine
Up-Regulation

Keywords

  • Adhesion molecules
  • Hippocampus
  • Synaptic plasticity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

N-methyl-d-aspartate receptor independent changes in expression of polysialic acid-neural cell adhesion molecule despite blockade of homosynaptic long-term potentiation and heterosynaptic long-term depression in the awake freely behaving rat dentate gyrus. / Rodríguez, Jose J.; Dallérac, Glenn M.; Tabuchi, Masashi; Davies, Heather A.; Colyer, Frances M.; Stewart, Michael G.; Doyère, Valérie.

In: Neuron Glia Biology, Vol. 4, No. 3, 01.12.2008, p. 169-178.

Research output: Contribution to journalArticle

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