The introduction of a useful new chromogenic substrate for the determination of elastase (EC 18.104.22.168) activity is described. N-acetyl-L-Ala-L-Ala-L-Ala-p-nitroanilide (AcAla3NA) is a new specific elastase substrate whose hydrolysis can be followed spectrophotometrically at 410 nm in a wide pH range. Its rate of hydrolysis by α-chymotrypsin (EC 22.214.171.124) and trypsin (EC 126.96.36.199.) is 0.02% and 0.001% respectively compared to its rate of hydrolysis by elastase. As little as 0.1 μg elastase/ml can be satisfactorily determined. At pH 8, Km = 0.88 mM and kcat = 11.9 sec-1.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 20 1973|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology