Myxoid Liposarcoma with t(12;16) (ql3;pll) Contains Site-specific Differences in Methylation Patterns Surrounding a Zinc-Finger Gene Mapped to the Breakpoint Region on Chromosome 12

Sylvie Paulien, Paola Dal Cin, Sheila Jani-Sait, Chandrika Sreekantaiah, Avery A. Sandberg, Robert M. Gemmill, Claude Turc-Carel, Paola Dal Cin, Stanley P.L. Leong, Bert Vogelstein, Kenneth W. Kinzler

Research output: Contribution to journalArticle

Abstract

The ql3 to ql5 region of human chromosome 12 is frequently and consistently rearranged in malignant and benign adipose tissue tumors as well as benign tumors of smooth muscle and salivary glands. A reciprocal translocation, (12;16) (ql3;pll), is characteristic of the myxoid subtype of liposarcoma, whereas translocations within 12ql3-14 are frequently observed in benign lipomas. We are using pulsed-field gel electrophoresis to study the 12ql3-ql4 region in order to detect and clone the respective translocation breakpoints in these tumors. The locus GLI, which encodes a zinc-finger protein, has been mapped to the same region as the myxoid liposarcoma breakpoint. Pulsed-field analysis of myxoid liposarcoma and lipoma DNA has allowed us to construct a 600-kilobase physical map surrounding the GLI locus, which shows that breakpoints in both types of tumor are outside this region. However, myxoid liposarcoma DNA samples contained altered restriction fragments detectable with GLI probes that were highly specific and reproducible from case to case. These altered fragments are due to highly specific and reproducible methylation differences that are unique to myxoid liposarcoma DNA. These methylation changes may prove to be useful clinically as a diagnostic tool to differentiate subtypes of liposarcoma.

Original languageEnglish (US)
Pages (from-to)7902-7907
Number of pages6
JournalCancer Research
Volume50
Issue number24
StatePublished - Dec 15 1990

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Paulien, S., Dal Cin, P., Jani-Sait, S., Sreekantaiah, C., Sandberg, A. A., Gemmill, R. M., Turc-Carel, C., Dal Cin, P., Leong, S. P. L., Vogelstein, B., & Kinzler, K. W. (1990). Myxoid Liposarcoma with t(12;16) (ql3;pll) Contains Site-specific Differences in Methylation Patterns Surrounding a Zinc-Finger Gene Mapped to the Breakpoint Region on Chromosome 12. Cancer Research, 50(24), 7902-7907.